FastQCFastQC Report
Wed 9 Mar 2022
H7L2KDMXY_n01_CIVR_Y3_P1_G3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7L2KDMXY_n01_CIVR_Y3_P1_G3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20045975
Sequences flagged as poor quality0
Sequence length151
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAGACTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCAA1641790.819012295485752No Hit
CACAGACTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACC1587030.7916950909097711No Hit
CTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCAACGTC1518030.7572742158962086No Hit
GCAGAATCCAGATGCTCAAGGCCCTTCATAATATCCCCCAGTTTAGTAGT759810.37903369629065187No Hit
AGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCAACGTCAAGGC718250.35830135476074376No Hit
GTCAACTTCAAGCTCCTAAGCCACTGCCTGCTGGTGACCCTGGCCGCCCA671850.33515456344727557No Hit
CAACTGTGTTCACTAGCAACCTCAAACAGACACCATGGTGCACCTGACTC618130.3083561662628034No Hit
GCGGGAAGTAGGTCTTGGTGGTGGGGAAGGACAGGAACATCCTCTCCAGG572770.28572818234084396No Hit
CGCGCACGCTGGCGAGTATGGTGCGGAGGCCCTGGAGAGGATGTTCCTGT503380.2511127545554656No Hit
GTTCACTAGCAACCTCAAACAGACACCATGGTGCACCTGACTCCTGAGGA492690.24578001319466874No Hit
CACAACTGTGTTCACTAGCAACCTCAAACAGACACCATGGTGCACCTGAC467420.23317399128752778No Hit
CCAAGACCTACTTCCCGCACTTCGACCTGAGCCACGGCTCTGCCCAGGTT455430.227192740687345No Hit
GGAACGGCTACCGAGGCTCCAGCTTAACGGTATTTGGAGGTCAGCACGGT451360.22516240791480585No Hit
GTCAGCACGGTGCTCACAGAAGCCAGGAACTTGTCCAGGGAGGCGTGCAC441660.22032353128246443No Hit
CGGCGCGCACGCTGGCGAGTATGGTGCGGAGGCCCTGGAGAGGATGTTCC437940.21846779715129847No Hit
CGGCTACCGAGGCTCCAGCTTAACGGTATTTGGAGGTCAGCACGGTGCTC402380.20072857518778708No Hit
CGGCAAGAAGGTGGCCGACGCGCTGACCAACGCCGTGGCGCACGTGGACG399500.19929187779591664No Hit
GGCGTGCACCGCAGGGGTGAACTCGGCGGGGAGGTGGGCGGCCAGGGTCA364470.1818170480607703No Hit
CTTGAAGTTGACCGGGTCCACCCGAAGCTTGTGCGCGTGCAGGTCGCTCA361670.18042025892978517No Hit
GTCAAGGCCGCCTGGGGTAAGGTCGGCGCGCACGCTGGCGAGTATGGTGC358980.17907834365751726No Hit
CGCACGTGGACGACATGCCCAACGCGCTGTCCGCCCTGAGCGACCTGCAC349950.17457369871009018No Hit
CGGAGGCCCTGGAGAGGATGTTCCTGTCCTTCCCCACCACCAAGACCTAC348260.1737306366988884No Hit
GCCAGGGTCACCAGCAGGCAGTGGCTTAGGAGCTTGAAGTTGACCGGGTC345600.17240368702445255No Hit
GTGCACCGCAGGGGTGAACTCGGCGGGGAGGTGGGCGGCCAGGGTCACCA345200.17220414572002607No Hit
GTTGGACTTAGGGAACAAAGGAACCTTTAATAGAAATTGGACAGCAAGAA340020.16962008582770358No Hit
GGATGTTCCTGTCCTTCCCCACCACCAAGACCTACTTCCCGCACTTCGAC328000.16362386962968875No Hit
CTTCAAGCTCCTAAGCCACTGCCTGCTGGTGACCCTGGCCGCCCACCTCC313820.1565501303877711No Hit
GGGAGGTGGGCGGCCAGGGTCACCAGCAGGCAGTGGCTTAGGAGCTTGAA312280.15578189636572928No Hit
AAGAAAGCGAGCTTAGTGATACTTGTGGGCCAGGGCATTAGCCACACCAG310670.15497874261541283No Hit
CGGCGGGGAGGTGGGCGGCCAGGGTCACCAGCAGGCAGTGGCTTAGGAGC310360.15482409810448233No Hit
GTCGAAGTGCGGGAAGTAGGTCTTGGTGGTGGGGAAGGACAGGAACATCC309930.1546095912022239No Hit
GCGACCTGCACGCGCACAAGCTTCGGGTGGACCCGGTCAACTTCAAGCTC307600.1534472631039398No Hit
CGCGCACAAGCTTCGGGTGGACCCGGTCAACTTCAAGCTCCTAAGCCACT300610.1499602788090876No Hit
GTGAACTCGGCGGGGAGGTGGGCGGCCAGGGTCACCAGCAGGCAGTGGCT294680.14700207897096548No Hit
GCCGGTGCAAGGAGGGGAGGAGGGCCCGTTGGGAGGCCCAGCGGGCAGGA283630.1414897504361848No Hit
GAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCAACGTCAAGG282570.14096096597945473No Hit
GGACGACATGCCCAACGCGCTGTCCGCCCTGAGCGACCTGCACGCGCACA281240.1402974911422368No Hit
GGCGGCCAGGGTCACCAGCAGGCAGTGGCTTAGGAGCTTGAAGTTGACCG279000.13918005983744866No Hit
GGCATGTCGTCCACGTGCGCCACGGCGTTGGTCAGCGCGTCGGCCACCTT277000.13818235331531642No Hit
GCGGAGGCCCTGGAGAGGATGTTCCTGTCCTTCCCCACCACCAAGACCTA273100.13623682559715855No Hit
CAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCAACGTCAA272220.13579783472742035No Hit
TGGGCATGTCGTCCACGTGCGCCACGGCGTTGGTCAGCGCGTCGGCCACC266420.13290448581323683No Hit
CTTGGACCCAGAGGTTCTTTGAGTCCTTTGGGGATCTGTCCACTCCTGAT264440.1319167563563259No Hit
GTCCAGGTGAGCCAGGCCATCACTAAAGGCACCGAGCACTTTCTTGCCAT263120.1312582700517186No Hit
GGAGGGCCCGTTGGGAGGCCCAGCGGGCAGGAGGAACGGCTACCGAGGCT262510.13095396956246827No Hit
CCACCATGGTGCTGTCTCCTGCCGACAAGACCAACGTCAAGGCCGCCTGG261880.13063969200799663No Hit
CCATGGTGCTGTCTCCTGCCGACAAGACCAACGTCAAGGCCGCCTGGGGT261170.13028550619263968No Hit
CGGGGAGGTGGGCGGCCAGGGTCACCAGCAGGCAGTGGCTTAGGAGCTTG252150.12578584977782323No Hit
GGGCGGCCAGGGTCACCAGCAGGCAGTGGCTTAGGAGCTTGAAGTTGACC251010.12521715706020783No Hit
CAGGCAGTGGCTTAGGAGCTTGAAGTTGACCGGGTCCACCCGAAGCTTGT246940.12318682428766872No Hit
GCCCAACGCGCTGTCCGCCCTGAGCGACCTGCACGCGCACAAGCTTCGGG246160.12279771874403715No Hit
GGCAAAGGTGCCCTTGAGGTTGTCCAGGTGAGCCAGGCCATCACTAAAGG245170.12230385401558169No Hit
GGCCGGTGCAAGGAGGGGAGGAGGGCCCGTTGGGAGGCCCAGCGGGCAGG243940.12169026450447036No Hit
GGGGAGGTGGGCGGCCAGGGTCACCAGCAGGCAGTGGCTTAGGAGCTTGA243290.12136600988477736No Hit
GGGAGGAGGGCCCGTTGGGAGGCCCAGCGGGCAGGAGGAACGGCTACCGA236430.11794387651386376No Hit
ATTTGGAGGTCAGCACGGTGCTCACAGAAGCCAGGAACTTGTCCAGGGAG227100.11328957558811681No Hit
CTCAGGTCGAAGTGCGGGAAGTAGGTCTTGGTGGTGGGGAAGGACAGGAA225520.11250138743563234No Hit
GGTATTTGGAGGTCAGCACGGTGCTCACAGAAGCCAGGAACTTGTCCAGG225200.11234175439209118No Hit
GTGAATTCTTTGCCAAAGTGATGGGCCAGCACACAGACCAGCACGTTGCC223690.11158848596788133No Hit
CCTGTCCTTCCCCACCACCAAGACCTACTTCCCGCACTTCGACCTGAGCC223040.11126423134818836No Hit
TGGTGCTGTCTCCTGCCGACAAGACCAACGTCAAGGCCGCCTGGGGTAAG222210.11085018314150347No Hit
CGAGGCTCCAGCTTAACGGTATTTGGAGGTCAGCACGGTGCTCACAGAAG219770.10963298118450211No Hit
ACAGACTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCA218730.10911417379299336No Hit
CAGCACGGTGCTCACAGAAGCCAGGAACTTGTCCAGGGAGGCGTGCACCG218300.10889966689073492No Hit
TCCTAAGCCACTGCCTGCTGGTGACCCTGGCCGCCCACCTCCCCGCCGAG218150.10882483890157499No Hit
GTGCTGTCTCCTGCCGACAAGACCAACGTCAAGGCCGCCTGGGGTAAGGT213990.10674960933553992No Hit
CTCAAGGGCACCTTTGCCACACTGAGTGAGCTGCACTGTGACAAGCTGCA213940.10672466667248663No Hit
CTGGAGAGGATGTTCCTGTCCTTCCCCACCACCAAGACCTACTTCCCGCA206250.10288848509488813No Hit
GGAGGAGGGCCCGTTGGGAGGCCCAGCGGGCAGGAGGAACGGCTACCGAG206210.10286853096444547No Hit
CGTGGATGAAGTTGGTGGTGAGGCCCTGGGCAGGCTGCTGGTGGTCTACC205690.1026091272686911No Hit
CGGGAAGTAGGTCTTGGTGGTGGGGAAGGACAGGAACATCCTCTCCAGGG205390.10245947129037127No Hit
GGGTAAGGTCGGCGCGCACGCTGGCGAGTATGGTGCGGAGGCCCTGGAGA205250.10238963183382199No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)