FastQCFastQC Report
Wed 9 Mar 2022
H7L2KDMXY_n01_CIVR_Y3_P1_D6.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7L2KDMXY_n01_CIVR_Y3_P1_D6.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11607
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTACAGCATCTCGTAT1521.3095545791332817TruSeq Adapter, Index 22 (97% over 38bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTACAGCATCGCGTAT990.8529335745670715TruSeq Adapter, Index 22 (97% over 38bp)
ATATCTCTTATGAACTCCGGCGTGCAACAGAGGATCCATCGAGGGCCTTA220.18954079434823815No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTACAGCATCTCGTA200.17230981304385284TruSeq Adapter, Index 22 (97% over 38bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTACAGCATCTCGGAT170.1464633410872749TruSeq Adapter, Index 22 (97% over 38bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTACAGCATCGCGTA160.1378478504350823TruSeq Adapter, Index 22 (97% over 38bp)
GCCTTAATATCTCTTATGAACTCCGGCGTGCAACAGAGGATCCATCGAGG150.12923235978288963No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTACAGCATCTCGTTT130.11200137847850436TruSeq Adapter, Index 22 (97% over 38bp)
CCTTAATATCTCTTATGAACTCCGGCGTGCAACAGAGGATCCATCGAGGG120.10338588782631171No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)