FastQCFastQC Report
Tue 25 Jul 2017
H7KNHBGX3_n01_mb36.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7KNHBGX3_n01_mb36.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences40529767
Sequences flagged as poor quality0
Sequence length75
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGG6335471.5631646735102127TruSeq Adapter, Index 3 (100% over 63bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC841100.064.24032644
CTCGTAT832900.064.2017642
TCGTATG820500.064.1755343
TATGCCG840900.064.1691346
TCTCGTA835900.064.06206541
TGCCGTC829850.063.7557848
GCCGTCT819250.063.5532849
ATGCCGT834050.063.42629647
CCGTCTT839550.063.06854650
ATCTCGT861650.063.02436440
CGTCTTC832200.063.01164251
CATCTCG867550.062.98148339
CGTCTGA976700.061.6477716
ACACGTC991550.061.03712513
ACGTCTG989900.060.9240515
GTATGCC895350.060.6679145
CACGTCT999900.060.46192614
GCACACG1029800.058.87714811
CACACGT1028500.058.87470212
CTTAGGC1022650.057.5851333
Produced by FastQC (version 0.11.2)