FastQCFastQC Report
Tue 25 Jul 2017
H7KNHBGX3_n01_mb35.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7KNHBGX3_n01_mb35.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences46019399
Sequences flagged as poor quality0
Sequence length75
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGG24460715.31530409599656TruSeq Adapter, Index 1 (100% over 63bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAATGG519340.11285240817682125TruSeq Adapter, Index 1 (100% over 63bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCGTCT3014800.064.8860449
TATGCCG3018450.064.8852246
TGCCGTC3017450.064.8757948
GATCTCG2976450.064.8418839
CGTATGC3022350.064.8068544
ATGCCGT3022650.064.79613547
TCGTATG3022800.064.7915643
CTCGTAT2997350.064.7672842
CACGATC2973850.064.7585936
TCTCGTA2990250.064.74345441
CCGTCTT3022300.064.7421550
CGATCTC2978300.064.7418238
CGTCTTC3020550.064.7394751
ACGATCT2979500.064.6681437
TCACGAT3011050.064.5107735
ATCTCGT2998950.064.4336940
ATCACGA3029100.064.35387434
CGTCTGA3094300.064.3157716
CATCACG3041750.064.2462933
ACACGTC3112700.064.1978513
Produced by FastQC (version 0.11.2)