FastQCFastQC Report
Tue 25 Jul 2017
H7KNHBGX3_n01_mb34.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7KNHBGX3_n01_mb34.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences43164969
Sequences flagged as poor quality0
Sequence length75
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGG492472211.409071091884718TruSeq Adapter, Index 12 (100% over 63bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAG834620.19335586688362963TruSeq Adapter, Index 12 (100% over 63bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAATGG714440.1655138452665169TruSeq Adapter, Index 12 (100% over 63bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAATG526430.12195769212761394TruSeq Adapter, Index 12 (100% over 63bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCTTC5960450.066.57603551
CCGTCTT5999000.066.5573350
GCCGTCT5969550.066.508849
TGCCGTC6019700.066.4403948
ATGCCGT6026450.066.439947
CTCGTAT6029050.066.320742
TATGCCG6089650.066.2830646
TCTCGTA6040200.066.2444641
CGTATGC6102600.066.2246444
TCGTATG6090100.066.1781743
AATCTCG6087700.066.0401739
ATCTCGT6083100.066.0148440
GATCGGA6343800.065.997211
ATCGGAA6418800.065.97622
GTATGCC6156700.065.76446545
TCGGAAG6430400.065.7506263
ACACGTC6394800.065.5775813
CGTCTGA6385700.065.5359516
ACGTCTG6390950.065.5063215
GCTTGAA6004900.065.4878759
Produced by FastQC (version 0.11.2)