FastQCFastQC Report
Tue 25 Jul 2017
H7KNHBGX3_n01_mb33.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7KNHBGX3_n01_mb33.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences40700878
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGG6272021.5410036117648371TruSeq Adapter, Index 7 (100% over 63bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT887100.065.6301642
TCTCGTA892000.065.5211141
TCGTATG878950.065.4144543
CGTATGC938450.065.3768944
ATGCCGT900850.065.2597447
TATGCCG948850.064.87443546
TGCCGTC924950.064.6783848
GCCGTCT896950.064.5627949
CCGTCTT913250.064.3170650
ATCTCGT947600.064.2250840
CGTCTTC908500.063.92038351
GATCGGA1161250.063.664781
CATCTCG978250.063.61632539
CGTCTGA1168350.063.4988216
ATCGGAA1177900.063.4049872
ACACGTC1175000.063.3402613
ACGTCTG1176250.063.1405115
GTATGCC993350.062.485945
CACGTCT1190650.062.4769914
TCGGAAG1206600.061.8710373
Produced by FastQC (version 0.11.2)