FastQCFastQC Report
Tue 25 Jul 2017
H7KNHBGX3_n01_ba86.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7KNHBGX3_n01_ba86.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences37005137
Sequences flagged as poor quality0
Sequence length75
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGG2713460.7332657625345368TruSeq Adapter, Index 6 (100% over 63bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT341750.063.1839642
TCTCGTA342500.063.1463341
TCGTATG343650.062.99524743
CGCCAAT345250.062.69230333
CGTATGC346000.062.62705244
ATGCCGT346400.062.4744747
TATCTCG351250.061.4944739
TGCCGTC351800.061.38802348
TATGCCG354300.061.1302146
GTCACGC356200.061.123529
GCCGTCT352850.061.06838249
ACGCCAA357750.060.71386332
CCGTCTT355250.060.6850350
ATCTCGT359750.060.18551340
CGTCTGA370650.059.2557716
CGTCTTC363000.059.24661651
TCACGCC369600.058.8419530
ACACGTC379900.057.9394213
AGTCACG376500.057.84611528
CACGCCA376150.057.78996331
Produced by FastQC (version 0.11.2)