FastQCFastQC Report
Tue 25 Jul 2017
H7KNHBGX3_n01_ba85.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7KNHBGX3_n01_ba85.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences44875183
Sequences flagged as poor quality0
Sequence length75
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGG1975370.4401920767654585TruSeq Adapter, Index 5 (100% over 63bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT265200.062.29893542
TCGTATG299200.062.27609643
CGTATGC309400.062.14089644
TCTCGTA266550.062.07407841
ATGCCGT305950.061.36363247
GATCTCG274800.060.74992439
TATGCCG316250.060.69613346
TGCCGTC309700.060.44244848
GCCGTCT308400.059.65696349
CCGTCTT318350.058.82182350
ATCTCGT284200.058.5588440
CGTCTTC322300.057.44785351
CGTCTGA372450.055.22782516
ACGTCTG384050.053.51543815
ACACGTC390800.052.6954613
GTATGCC373350.051.89436745
CACGTCT409300.050.3392414
CACACGT443550.046.66227312
GCACACG444800.046.5154211
GTCGTAT52600.046.501842
Produced by FastQC (version 0.11.2)