FastQCFastQC Report
Tue 25 Jul 2017
H7KNHBGX3_n01_ba84.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7KNHBGX3_n01_ba84.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences40159227
Sequences flagged as poor quality0
Sequence length75
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGG2438110.6071107892589666TruSeq Adapter, Index 4 (100% over 63bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT317450.061.71727442
TCGTATG322950.061.5742143
TCTCGTA319550.061.21451641
ATGCCGT324850.061.11780547
CGTATGC327000.060.71656444
AATCTCG325150.060.12824639
TATGCCG333000.059.7878146
TGCCGTC332650.059.6224948
GCCGTCT333450.059.40716649
CCGTCTT335350.059.07058350
ATCTCGT334200.058.54150840
CGTCTTC343500.057.66869451
CGTCTGA374250.053.75494816
GTATGCC378150.052.77757345
ACGTCTG386850.052.102615
ACACGTC398550.050.7100513
CACGTCT413150.048.91865514
ACCAATC423050.046.20501736
GACCAAT433150.045.73293335
CACACGT444900.045.6210812
Produced by FastQC (version 0.11.2)