FastQCFastQC Report
Tue 25 Jul 2017
H7KNHBGX3_n01_ba83.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7KNHBGX3_n01_ba83.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences39374378
Sequences flagged as poor quality0
Sequence length75
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGG3630820.9221275825614312TruSeq Adapter, Index 2 (100% over 63bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT490400.064.8757142
TCGTATG482700.064.8741843
TCTCGTA492450.064.78770441
CGATGTA569000.064.7410234
CGTATGC511050.064.6775844
ACCGATG573300.064.38196632
ATGCCGT497250.064.2586947
CACCGAT577300.064.09730531
CCGATGT575900.063.9955433
TATCTCG513250.063.90302739
TGCCGTC501450.063.8513648
TATGCCG519900.063.78838346
ATCTCGT512800.063.25943440
GCCGTCT498150.063.0278649
CCGTCTT508900.062.83542650
GTCACCG591450.062.58732229
CGTCTTC507150.062.31061651
TCACCGA596900.062.03328330
CGTCTGA619200.060.23264716
TCGCGTA37050.059.8728141
Produced by FastQC (version 0.11.2)