FastQCFastQC Report
Tue 25 Jul 2017
H7KNHBGX3_n01_ba67.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7KNHBGX3_n01_ba67.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences35428163
Sequences flagged as poor quality0
Sequence length75
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGG1004790.28361335020390416TruSeq Adapter, Index 11 (100% over 63bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT140350.056.87961242
TCGTATG142300.056.4395843
TCTCGTA142300.056.02743541
CGTATGC146700.054.86443744
ATGCCGT148950.053.8966247
CGGCTAC149600.053.0164533
ACGGCTA152150.052.85347432
TATGCCG151950.052.80989546
TGCCGTC157900.050.8636148
ATCTCGT157550.050.60427540
CCGTCTT160650.049.97159250
GCCGTCT163150.049.10013249
CATCTCG163000.048.9756539
CGTCTGA172950.047.61693616
CGTCTTC168550.047.60866551
TCACGGC170600.047.2184530
ACACGTC176700.046.58706313
GTCACGG174400.046.5260929
AGTCACG176450.046.06381628
ACGTCTG182700.045.22738315
Produced by FastQC (version 0.11.2)