FastQCFastQC Report
Tue 25 Jul 2017
H7KNHBGX3_n01_ba66.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7KNHBGX3_n01_ba66.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences44827461
Sequences flagged as poor quality0
Sequence length75
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGG586450.13082382693947353TruSeq Adapter, Index 10 (100% over 63bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTCGTA111600.053.32519541
CTCGTAT111500.053.2802642
CGTATGC120100.052.94066244
TCGTATG107950.052.50769443
TCGCGTA18700.052.3943341
ATGCCGT120250.051.2675647
TATGCCG131900.049.7734446
TATCTCG131200.049.14525239
TGCCGTC129300.048.87993648
TATCGCG31300.048.2765139
GCCGTCT127750.047.5556549
CGTCTGA189550.047.3606516
CCGTCTT137500.046.91838550
CGCGTAT20750.046.8855542
ATCGCGT25450.046.49576640
ATCTCGT135700.046.2190640
ACGTCTG198800.045.29634515
ACACGTC201350.044.824913
CGTCTTC144200.044.11622651
GATCGGA211450.042.8053361
Produced by FastQC (version 0.11.2)