FastQCFastQC Report
Fri 8 Jul 2016
H7KMHBGXY_n01_apex24.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7KMHBGXY_n01_apex24.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14342162
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGTAGCATCTCGTATGC1550011.0807366420766966TruSeq Adapter, Index 22 (97% over 37bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG171950.065.36597446
GTATGCC181600.062.04405645
CGTATGC181450.061.8050144
ATGCCGT183600.061.06581547
TCGTATG190300.059.07803743
TGCCGTC189700.058.77035548
GCCGTCT191650.057.80700349
CTCGTAT196200.056.80172742
GGTAGCA213850.053.82599334
ACGGTAG217050.053.452432
CATCTCG215650.051.7597739
TCTCGTA217500.051.30342541
GTCACGG228350.050.848429
AGTCACG228650.050.83292828
AAAAGGG200350.050.5815370
GTAGCAT226950.050.57620635
CGGTAGC230400.050.02103833
CCGTCTT228500.048.6990850
ATCTCGT236400.047.27576440
TAGCATC250450.045.80040436
Produced by FastQC (version 0.11.2)