FastQCFastQC Report
Fri 8 Jul 2016
H7KMHBGXY_n01_apex23.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7KMHBGXY_n01_apex23.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13940597
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGTGGATCTCGTATGC1161500.833178091296951TruSeq Adapter, Index 7 (97% over 36bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG133450.064.7424246
CGTATGC144750.059.82858344
ATGCCGT143350.059.80737747
GTATGCC145100.059.78287545
TCGTATG149450.056.6589443
TGCCGTC151200.056.19333348
GCCGTCT150850.055.5817949
CTCGTAT152850.054.82670642
CGAGTGG175200.051.73689333
ACGAGTG189100.048.60104832
TCTCGTA175900.047.44327541
CACGAGT194800.047.2886131
AAAAGGG169900.046.6841870
AGTGGAT195550.046.02643235
CCGTCTT190650.044.3826950
GTCACGA212200.043.23813629
ATCTCGT193500.043.2363640
TGAAAAA208650.043.01613662
GTGGATC205850.042.88813836
TCACGAG215500.042.74933230
Produced by FastQC (version 0.11.2)