FastQCFastQC Report
Fri 8 Jul 2016
H7KMHBGXY_n01_apex22.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7KMHBGXY_n01_apex22.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20891016
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGTACGATCTCGTATGC1894180.9066959692147093TruSeq Adapter, Index 22 (98% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG218450.064.7487346
GTATGCC232750.060.85907445
CGTATGC232350.060.78093744
ATGCCGT237350.059.6223347
TCGTATG245700.057.33561343
TGCCGTC247500.057.19148648
CGTACGA252100.055.75312434
GCCGTCT253250.055.71346349
CTCGTAT250150.055.1550442
TACGATC253100.053.94819636
CCGTACG266100.053.1094633
GTACGAT269650.051.76907335
ACCGTAC291400.048.606732
TCTCGTA286950.047.83778441
AAAAGGG262200.047.37001870
CCGTCTT308900.045.97147450
ACGATCT301150.045.37328737
ATCTCGT310050.044.10438540
GATCTCG315350.043.4409139
TGAAAAA329150.043.42768562
Produced by FastQC (version 0.11.2)