FastQCFastQC Report
Fri 8 Jul 2016
H7KMHBGXY_n01_apex21.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7KMHBGXY_n01_apex21.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27707048
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTTCGATCTCGTATGC4395261.5863328348801358TruSeq Adapter, Index 21 (98% over 50bp)

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG484450.066.38991546
GTATGCC502350.064.0843145
CGTATGC504300.063.8129344
ATGCCGT506350.063.6083547
TGCCGTC520800.061.79712348
TCGTATG523100.061.49276743
GCCGTCT526650.061.04478549
CTCGTAT523450.059.97456742
CGTTTCG572700.056.117733
TCTCGTA562400.055.4350841
CCGTCTT594300.054.17860850
AAAAGGG519600.053.86165670
TTCGATC591400.052.44217736
TTTCGAT609850.052.01167735
ATCTCGT597600.051.9356240
GTTTCGA620250.051.53306234
TGAAAAA633100.051.2294362
GATCTCG606950.051.1643839
CACGTTT635600.050.7044231
GTCACGT640900.050.29792429
Produced by FastQC (version 0.11.2)