FastQCFastQC Report
Fri 8 Jul 2016
H7KMHBGXY_n01_apex20.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7KMHBGXY_n01_apex20.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14395593
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGGCCATCTCGTATGC1541841.0710500081518004TruSeq Adapter, Index 20 (98% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG184650.065.2652946
GTATGCC193850.062.47123745
CGTATGC196250.061.49181444
ATGCCGT195600.061.27173647
TGCCGTC200850.059.16533748
TCGTATG201350.058.69947443
GCCGTCT200350.058.38731849
CTCGTAT199750.056.84000842
CGTGGCC231350.054.67150533
AAAAGGG211850.051.8079870
ACGTGGC245400.051.75583332
GGCCATC226700.051.73827436
TCTCGTA222050.050.7535241
CATCTCG222750.050.68844639
GTGGCCA254050.049.85500334
CACGTGG257650.049.44674731
CCGTCTT238500.049.40009350
TCACGTG262700.048.45944230
TGAAAAA262600.047.69117462
TGGCCAT262300.047.41522235
Produced by FastQC (version 0.11.2)