FastQCFastQC Report
Fri 8 Jul 2016
H7KMHBGXY_n01_apex19.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7KMHBGXY_n01_apex19.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16197615
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAAAATCTCGTATGC2262021.3965142399050725TruSeq Adapter, Index 19 (98% over 50bp)

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG255400.065.5867846
GTATGCC264550.063.67270745
CGTATGC267400.062.62632444
ATGCCGT269300.061.9158247
TGCCGTC272200.060.62700748
TCGTATG275000.060.38716543
GCCGTCT275150.059.37926549
CTCGTAT278600.059.3183642
AAAAGGG278050.054.47153570
TCTCGTA309200.053.6627641
CCGTCTT316400.051.9702550
AATCTCG323600.051.3288739
TGAAAAA338050.050.55327262
ATCTCGT333800.049.76040340
ACGTGAA352100.049.29099332
CGTGAAA354000.048.92711633
AAAAAGG319700.047.4562969
CTGCTTG343000.047.3787157
GAAAAAA364600.047.05042363
TTGAAAA360650.046.42278361
Produced by FastQC (version 0.11.2)