FastQCFastQC Report
Fri 8 Jul 2016
H7KMHBGXY_n01_apex18.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7KMHBGXY_n01_apex18.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18983074
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCCGCATCTCGTATGC2305881.2147031613531085TruSeq Adapter, Index 18 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG255450.066.0132346
GTATGCC271250.062.78528245
CGTATGC270900.062.60542744
ATGCCGT270750.062.02435347
TGCCGTC279800.059.7060248
TCGTATG281000.059.54517743
GTCCGCA298850.059.0695434
CTCGTAT284000.058.96577542
GCCGTCT283050.058.6007549
CGTCCGC304250.058.28616333
ACGTCCG315350.056.20170632
TCCGCAT325800.053.84593635
CGCATCT317950.053.4071537
TCTCGTA315000.053.16279241
AAAAGGG289900.052.4377870
CATCTCG322050.052.21630539
CCGCATC334900.051.9512436
CCGTCTT332200.050.18379650
TCACGTC356200.049.8205430
GTCACGT356550.049.7084229
Produced by FastQC (version 0.11.2)