FastQCFastQC Report
Fri 8 Jul 2016
H7KMHBGXY_n01_apex17.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7KMHBGXY_n01_apex17.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17449100
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTAGAGATCTCGTATGC3052511.7493796241639972TruSeq Adapter, Index 3 (97% over 37bp)

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG341000.066.8966946
CGTATGC354350.064.3818344
GTATGCC355150.064.2678445
ATGCCGT356500.064.0177747
TCGTATG364450.062.52951443
TGCCGTC366550.062.30125448
CTCGTAT365650.061.7790342
GCCGTCT371900.061.35832249
TCTCGTA395750.057.00950241
AAAAGGG364200.055.2697270
CCGTCTT413950.055.2691150
CGTAGAG421450.054.3162333
CACGTAG424000.054.05931531
ATCTCGT419950.053.6323240
GATCTCG421850.053.4577339
TGAAAAA430850.053.3680762
TCACGTA430650.053.25213230
ACGTAGA435650.052.73131632
GTCACGT441800.051.91352529
CTGCTTG441200.051.63212657
Produced by FastQC (version 0.11.2)