FastQCFastQC Report
Fri 8 Jul 2016
H7KMHBGXY_n01_apex16.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7KMHBGXY_n01_apex16.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17788483
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTCCATCTCGTATGC1756760.9875828084946872TruSeq Adapter, Index 16 (98% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG207700.065.8248846
GTATGCC220700.062.10437845
ATGCCGT222100.061.19470647
CGTATGC224050.061.0165244
TGCCGTC227650.059.25769848
TCGTATG232750.058.1043743
GCCGTCT232600.057.57602749
CTCGTAT236050.056.47694442
CCCGTCC253700.055.0562633
ACCCGTC268250.052.2271332
TCTCGTA260900.050.547841
CCGTCCA276550.050.35521734
CACCCGT278850.050.3316531
CATCTCG264100.049.59113739
TCACCCG285450.049.06114230
AAAAGGG244450.048.71961670
CCGTCTT280200.048.08287450
GTCACCC296300.047.35209329
TGAAAAA304100.045.83081462
ATCTCGT287300.045.8177140
Produced by FastQC (version 0.11.2)