FastQCFastQC Report
Fri 8 Jul 2016
H7KMHBGXY_n01_apex15.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7KMHBGXY_n01_apex15.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20808659
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATGTCAATCTCGTATGC2913541.4001575017400207TruSeq Adapter, Index 15 (98% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG327100.065.9838346
GTATGCC340650.063.48093845
CGTATGC342550.062.9634344
ATGCCGT345250.062.53558747
TCGTATG354700.060.8458243
TGCCGTC356800.060.42370648
GCCGTCT360300.059.70105749
CTCGTAT356400.059.31870742
TCTCGTA391700.053.95503641
AAAAGGG358000.053.36779870
CCGTCTT409750.052.71027450
GTCAATC406050.052.06735636
AATCTCG411600.051.1935339
ATCTCGT414800.050.92500340
ACATGTC427000.050.89963532
CACATGT439700.049.53581631
CATGTCA441100.049.3037433
TGAAAAA443650.048.94592362
CTGCTTG445200.048.5870457
TCACATG454650.047.9483330
Produced by FastQC (version 0.11.2)