FastQCFastQC Report
Fri 8 Jul 2016
H7KMHBGXY_n01_apex14.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7KMHBGXY_n01_apex14.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21073020
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCATCTCGTATGC2079230.9866787010120049TruSeq Adapter, Index 14 (98% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG245650.064.90176446
GTATGCC259100.061.78679745
CGTATGC261300.061.06397644
ATGCCGT261500.060.84764547
TGCCGTC268600.059.1351648
GCCGTCT274350.057.5897749
TCGTATG276300.057.4571943
CTCGTAT275350.056.60067742
TCTCGTA310900.049.9148141
AAAAGGG290100.049.4664470
CATCTCG319800.048.30697339
CCGTCTT328550.048.19608750
ACAGTTC356250.045.9171532
TGAAAAA356200.045.4778862
ATCTCGT343400.045.07880440
AAAAAGG335150.043.11270569
CTGCTTG366800.043.05296757
TTGAAAA392900.040.96123561
GTCACAG400350.040.7895329
CACAGTT403700.040.6961431
Produced by FastQC (version 0.11.2)