FastQCFastQC Report
Fri 8 Jul 2016
H7KMHBGXY_n01_apex13.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7KMHBGXY_n01_apex13.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11952215
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAAATCTCGTATGC1887041.5788203274455823TruSeq Adapter, Index 13 (98% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG216450.065.46084646
GTATGCC225950.062.84400645
CGTATGC228400.062.15067344
ATGCCGT228050.062.0853447
TCGTATG233450.060.86668843
TGCCGTC233050.060.7535748
GCCGTCT234900.060.11173249
CTCGTAT234000.059.66246842
TCTCGTA254300.054.8722841
AAAAGGG233900.054.55607270
CCGTCTT263050.053.83902750
GTCAAAT271100.052.38386535
AATCTCG269150.051.88355339
ACAGTCA280800.051.11449432
ATCTCGT273700.050.982940
TGAAAAA280550.050.90272562
CACAGTC289450.049.7470831
CTGCTTG287650.049.143757
AAAAAGG260900.049.03426469
CAGTCAA293200.048.9875533
Produced by FastQC (version 0.11.2)