FastQCFastQC Report
Fri 8 Jul 2016
H7KMHBGXY_n01_apex12.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7KMHBGXY_n01_apex12.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14070261
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCTCGTATGC283220.20128979839108885TruSeq Adapter, Index 11 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG40700.053.5649646
CGTATGC48650.045.3119244
ATGCCGT49850.044.0842247
GTATGCC50950.043.68051545
CGGCTAC55250.039.9710933
TGCCGTC58400.037.9900448
TCGTATG60350.036.4116443
GGCTACA60750.036.35153234
ACGGCTA61000.036.2037132
GCCGTCT62850.034.91058349
CTCGTAT64650.034.2604542
GCTACAT71850.030.78172135
CACGGCT76550.029.17074631
GTCACGG77200.028.97438829
AGTCACG77200.028.93151328
CTACATC80000.027.60032336
TCACGGC86950.025.68294130
TCTCGTA88350.024.91161741
AAAAGGG81800.024.61411770
CATCTCG90200.024.28438839
Produced by FastQC (version 0.11.2)