FastQCFastQC Report
Fri 8 Jul 2016
H7KMHBGXY_n01_apex11.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7KMHBGXY_n01_apex11.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14120700
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG20550.040.86785546
GTATGCC28700.029.50480845
CGTATGC29200.028.51924744
ATGCCGT32350.025.52819447
TCGTATG38600.021.57414443
TGCCGTC38800.020.74347948
CTCGTAT40250.020.42901642
TATCTCG41650.020.33039739
GGCGCGC7800.020.18661138
GCCGTCT42950.018.49473649
CGCGCAC9450.017.03210840
GCTTATC61000.016.11937136
TAGCTTA67150.015.32261634
ACTAGCT67250.014.62330832
CACTAGC68950.014.36509131
AGCTTAT71350.013.92916835
GCGCGCA12000.013.41288139
TCACTAG76150.013.00801930
TCTCGTA65750.012.9317141
AAAAGGG65600.012.86521970
Produced by FastQC (version 0.11.2)