FastQCFastQC Report
Fri 8 Jul 2016
H7KMHBGXY_n01_apex10.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7KMHBGXY_n01_apex10.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16834072
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG15800.027.68441246
GGCGCGC8550.023.73638538
GCGCGCA11600.017.49540539
GTATGCC26150.016.9942345
CGTATGC27500.016.28663344
CGCGCAC12050.016.2612940
ATGCCGT33500.014.10173847
CGGGGGT6150.013.0920991
GCGCTAA14400.012.64608868
TGCGCTA15300.012.36023467
TCGTATG38550.012.07209243
CGCCAAT44500.011.6428231
GCGCACC18600.011.28739241
TGCCGTC39700.011.2825148
CCCGTCT21700.011.13131
CGCGCGA4501.6276681E-710.89110851
CCCCGGG16400.010.88976362
CGCGTGT11650.010.8176561
CCCGTGT18250.010.7419161
CATGCGC17650.010.7145765
Produced by FastQC (version 0.11.2)