FastQCFastQC Report
Fri 8 Jul 2016
H7KMHBGXY_n01_apex09.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7KMHBGXY_n01_apex09.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15031574
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG19000.035.72905346
CGTATGC29650.024.19162444
GTATGCC30250.023.36579745
ATGCCGT32450.020.81224347
GGCGCGC7500.019.12757338
TCGTATG37700.018.28343643
CTCGTAT45050.016.69857842
TGCCGTC39650.016.41535848
CGCGCAC9900.014.84396540
GCCGTCT44700.014.48268749
CGCGTAT10650.014.1329821
CAGGCCG11550.013.93496516
CGCGCAT8450.013.2558721
GCGCGCA12100.012.43427139
CGGGGGT7200.011.6679281
CGCGGAT17650.011.502661
AGGGGGG4903.770765E-911.4250544
ACGCCCG15500.011.29349723
CGGGCCC2802.3453406E-411.2512161
TCTCGTA67500.011.19659141
Produced by FastQC (version 0.11.2)