FastQCFastQC Report
Fri 8 Jul 2016
H7KMHBGXY_n01_apex08.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7KMHBGXY_n01_apex08.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13162328
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG16150.036.8346446
GTATGCC25650.024.28293245
CGTATGC25100.023.97747244
GGCGCGC8050.021.73315638
ATGCCGT28250.021.4294547
TGCCGTC33550.017.5228648
TCGTATG34350.017.31686443
CGCGCAC10200.017.15214740
GCGCGCA11250.016.17333239
GCCGTCT37150.016.0133349
CGGGGGT5900.014.8338161
CTCGTAT39700.014.71887542
CGCCAAT32650.013.0810171
CGGGCCC3751.2898454E-813.069581
CGCGTAT10550.012.6094471
CCCCGGG13800.012.18088662
TAGGCAT51800.012.16034435
ACTTAGG51400.011.98364632
TTAGGCA54500.011.87968134
CCCGGGG8500.011.5319821
Produced by FastQC (version 0.11.2)