FastQCFastQC Report
Fri 8 Jul 2016
H7KMHBGXY_n01_apex07.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7KMHBGXY_n01_apex07.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16371500
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATGC426540.2605381302873897TruSeq Adapter, Index 1 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG54700.055.656646
GTATGCC66350.046.25125545
CGTATGC67100.045.68074844
ATGCCGT71150.043.03461547
TGCCGTC75900.040.34140848
TCGTATG78000.039.20719543
GCCGTCT83000.036.932949
CTCGTAT82350.036.54139342
TCTCGTA108200.028.03768241
TCACGAT112350.027.69135
AAAAGGG102650.026.54075470
CCGTCTT123950.025.21144150
CACGATC123050.024.42795236
ACGATCT128800.023.82513237
ATCACGA137450.023.19598434
ATCTCGT131750.022.9197840
GATCTCG132250.022.91250239
CATCACG137900.022.61284833
TGAAAAA143800.021.84356562
GTCACAT143550.021.70275529
Produced by FastQC (version 0.11.2)