FastQCFastQC Report
Fri 8 Jul 2016
H7KMHBGXY_n01_apex06.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7KMHBGXY_n01_apex06.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15545850
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGC477610.3072266875082418TruSeq Adapter, Index 12 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG60650.057.75140446
GTATGCC71200.049.29004745
CGTATGC71700.048.89642344
ATGCCGT74500.046.92158547
TCGTATG77250.045.51906643
TGCCGTC83300.041.88134448
CTCGTAT83950.041.01124242
GCCGTCT89300.039.10655249
TCTCGTA110350.031.39000541
AAAAGGG104600.030.40100970
CCGTCTT126150.027.90512750
AATCTCG127850.027.33967239
ATCTCGT128550.027.19079840
GTAATCT134850.025.89445337
GTCACCT140600.025.57145129
TGTAATC141750.024.88217736
CTGCTTG148900.023.84139657
TGAAAAA151050.023.60364262
AAAAAGG136700.023.54509469
TTGTAAT152800.023.42845735
Produced by FastQC (version 0.11.2)