FastQCFastQC Report
Fri 8 Jul 2016
H7KMHBGXY_n01_apex05.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7KMHBGXY_n01_apex05.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15688645
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGC163450.10418363089992795TruSeq Adapter, Index 7 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG28550.046.5770146
CGTATGC40150.033.5531544
GTATGCC40700.033.2726345
ATGCCGT42850.031.03332147
TGCCGTC49900.026.22816748
TCGTATG50850.025.8044343
CTCGTAT53350.024.59538342
GCCGTCT54900.023.83952149
TCTCGTA77350.017.68777541
GGCGCGC7350.017.13835538
AAAAGGG82200.015.89028270
CATCTCG84600.015.63424139
CCGTCTT92250.014.49098950
ATCTCGT96800.013.98918340
CAGATCA110250.013.61781534
TGAAAAA116850.012.52701862
CAGTCAC123850.012.52203627
AAAAAGG107050.012.49708569
CGCGCAC10950.011.82345440
GGGCCGC7750.011.74117354
Produced by FastQC (version 0.11.2)