FastQCFastQC Report
Fri 8 Jul 2016
H7KMHBGXY_n01_apex04.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7KMHBGXY_n01_apex04.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14787402
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGC430690.2912546774612606TruSeq Adapter, Index 6 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG53650.058.1180546
GTATGCC64250.048.74394245
CGTATGC65050.048.51938644
ATGCCGT67850.046.2642647
TCGTATG72550.043.45536843
TGCCGTC76350.041.11382748
TATCTCG78250.040.11133239
CTCGTAT78550.039.91332242
GCCGTCT82350.038.20327449
GTCACGC82550.038.03699529
TCACGCC84250.037.43251430
CACGCCA88100.035.9930431
CGCCAAT95100.033.19458433
ACGCCAA95600.033.05769332
TCTCGTA106000.029.74232941
AGTCACG108500.029.29673828
AAAAGGG101350.027.77820670
CCGTCTT115300.027.55893950
CCAATAT115250.027.51026235
GCCAATA117100.027.01788334
Produced by FastQC (version 0.11.2)