FastQCFastQC Report
Fri 8 Jul 2016
H7KMHBGXY_n01_apex03.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7KMHBGXY_n01_apex03.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15154620
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGC379620.25049786797689416TruSeq Adapter, Index 5 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG55850.056.89161346
GTATGCC66800.047.98173545
CGTATGC67150.047.46843344
ATGCCGT68350.046.48730547
TCGTATG75550.041.86607443
TGCCGTC78100.040.235948
GCCGTCT80050.038.77490249
CTCGTAT73500.038.4640642
CACAGTG109900.030.15788833
AAAAGGG100450.029.21504270
TCTCGTA99700.028.5667241
ACACAGT118950.028.30483832
CACACAG119800.028.01738731
CCGTCTT119750.026.53402150
GATCTCG116450.024.69809339
ATCTCGT118300.024.19355640
TGAAAAA142100.023.75736662
AGTGATC125500.023.6717836
TCACACA142100.023.64691530
AAAAAGG127850.023.2569369
Produced by FastQC (version 0.11.2)