FastQCFastQC Report
Fri 8 Jul 2016
H7KMHBGXY_n01_apex02.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7KMHBGXY_n01_apex02.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18746011
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGC744460.3971298213790657TruSeq Adapter, Index 4 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG96300.058.64947546
GTATGCC106850.053.05385245
CGTATGC109500.051.67113544
ATGCCGT114800.049.4724647
TCGTATG121350.046.5391243
TGCCGTC121950.046.3713748
CTCGTAT123650.044.99453742
GCCGTCT127600.044.2639549
ACTGACC150150.038.2719932
CACTGAC156250.037.29470431
TCTCGTA155650.036.01389741
AAAAGGG148850.034.3937770
CCGTCTT173000.032.91126350
AGTCACT183900.032.1334428
AATCTCG175250.031.92611139
ATCTCGT183900.030.46231740
GTCACTG194300.030.33890229
GACCAAT196150.029.20487435
TGAAAAA203750.028.46066962
CTGCTTG203750.028.3333357
Produced by FastQC (version 0.11.2)