FastQCFastQC Report
Fri 8 Jul 2016
H7KMHBGXY_n01_apex01.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7KMHBGXY_n01_apex01.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20649168
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGC1281440.620577061506788TruSeq Adapter, Index 2 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG156700.062.97384646
CGTATGC169100.058.4132844
GTATGCC170850.058.30916245
ATGCCGT174550.055.9727547
TCGTATG180800.053.7241543
TGCCGTC182350.053.1187448
CTCGTAT183250.052.96781542
TATCTCG189900.051.49978339
GCCGTCT187500.051.0813149
CCGATGT222550.047.22465533
GATGTAT227450.046.14148735
ATGTATC236150.044.27515436
TCTCGTA221100.044.1375641
AAAAGGG212050.043.7967170
CGATGTA240550.043.6468434
GTATCTC234950.042.20567338
CCGTCTT237600.040.6198650
ACCGATG260600.040.58537732
TGTATCT247650.040.4228637
ATCTCGT252900.038.80882340
Produced by FastQC (version 0.11.2)