FastQCFastQC Report
Sat 5 Dec 2020
H7KKVBGXH_n02_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7KKVBGXH_n02_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16447421
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG9723045.911589421830937No Hit
TTTTTAAACAATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCG361460.2197669774489265No Hit
CCCTTATACTGGAGATCCTCCATACAGCCATGGAACAGGAACAGGATACA353920.2151826721040338No Hit
AATATATTCAATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCA286610.17425832293099325No Hit
GAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCACTCGCGAGATA277100.1684762614150875No Hit
AGTAGAAACAAGGCATTTTTTCATGAAGGACAAGTTAAATTCATTATTTT228640.13901267560427863No Hit
GGTCAAATATATTCAATATGGAGAGAATAAAAGAACTGAGAGATCTAATG205970.12522935966678303No Hit
GTAGAAACAAGGTCGTTTTTAAACAATTCGACACTAATTGATGGCCATCC201740.12265752788841486No Hit
CACTAAGACCACTGTGGACCATATGGCCATAATCAAAAAGTACACATCAG192150.11682682652800096No Hit
AGCAGGTACTGATCCAAAATGGAAGACTTTGTGCGACAATGCTTCAATCC188330.11450427395273703No Hit
CCTATATGAACTGCTAGGGAAAAATTTCTCGAATAGATTGCAGCACTTCT174710.10622334042522533No Hit
ATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCT171320.1041622270141927No Hit
TTTTTGGACAGTATGGATAGCAAATAGTAGCATTGCCACAACTACTTCAG167460.10181535451667467No Hit
TTCTTGATCCGTCCAGACTCGAAGTCGACCCTGGCATCAATCCGGGCCCT166920.1014870355662447No Hit
AAACAAGGTCGTTTTTAAACAATTCGACACTAATTGATGGCCATCCGAAT164490.10000960028930979No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAATTCG140800.048.075429
AGTAGAA157700.046.470241
CAGGTAC73050.044.0584143
GCAGGTA77950.040.903622
GTAGAAA180500.040.3472632
CCTTATA211200.037.8105432
CCCTTAT214300.037.6062621
ACTGATC93950.037.5673988
TTATACT237550.034.7850884
GTACTGA103500.034.595686
GGTACTG96600.034.294255
AGGTCGT86750.034.173476
CGAAAGC64100.034.038833
GGTCGTT86250.033.361167
ACAATTC219950.032.1921128
TAGAAAC248950.030.8645463
TATATTC173100.030.5697523
TATACTG299650.030.3365025
AGCGAAA68600.030.2659931
GGATAGC84000.029.945565
Produced by FastQC (version 0.11.5)