FastQCFastQC Report
Sat 5 Dec 2020
H7KKVBGXH_n02_S6_R2_H04_FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7KKVBGXH_n02_S6_R2_H04_FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4721302
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATATATGAGTAAACTTGGTCTGACAGTTACCAATGCTTAATCAGTGAG145990.309215551133988No Hit
TCCTTAGGCAGAACCCAACAGAAGAGCAAGCCGTGGATATATGCAAGGCT104950.2222903766799921No Hit
GTATTATCCCGTATTGACGCCGGGCAAGAGCAACTCGGTCGCCGCATACA103810.21987578850071443No Hit
GTATAGCATTGTAGGGTTCTGGGACCACTGAATTTTAACAGTTTCCCAGT99970.2117424388442002No Hit
GGATGTAATCATGGAAGTTGTTTTCCCTAACGAAGTGGGAGCCAGGATAC99820.2114247298732426No Hit
ATTATATTCAATATGGAAAGAATAAAAGAACTAAGAAATCTAATGTCGCA92240.1953698365408525No Hit
TTCATATACTGATGACCTCTTTATAGCCAACCTTTGTTCATGGCAGCCAG90100.1908371885551909No Hit
GGATAATACCGCGCCACATAGCAGAACTTTAAAAGTGCTCATCATTGGAA89160.18884621233719004No Hit
GCCTAAGGATGTCTACCATCCTAATTCCACCAATCTGTGTGCTGTGGCAC88910.18831669738559406No Hit
CTTCATATCCCTCATGCACTCTTATCTTCAATGTTTGAAGATTGCCCGTA87740.18583856741212487No Hit
GATGTATACTCCAGGAGGGGAAGTGAGGAATGATGATGTTGATCAAAGCT85750.18162362839742088No Hit
AACAAATACAGTTCATTATCCAAAAATCTACAAAACTTATTTTGAAAGAG85460.18100939105356956No Hit
CATATATACTTTAGATTGATTTAAAACTTCATTTTTAATTTAAAAGGATC83660.1771968834020785No Hit
GTATTTGTTATTGGTCCATTCCTATTCCACCATGTCACAGCCAGAGGTGA82110.17391389070218344No Hit
ATGCTATACAATAAAATGGAATTTGAACCATTTCAGTCTTTAGTACCTAA81700.17304548618156604No Hit
ATTACATCCTGTGCCTCCTTGGCACTGAGATCTGCATGACCAGGATTTAT81560.17274895780867225No Hit
CCCATATGTCCTTCCGAGTGAGAGACACAAAAAATTCCAACACACTATTG80870.17128749654226738No Hit
ACATGGGGGATCATGTAACTCGCCTTGATCGTTGGGAACCGGAGCTGAAT79470.16832221281332987No Hit
CTTTATAGCCAACCTTTGTTCATGGCAGCCAGCATATGGGCATATGTTGC78930.16717846051788257No Hit
TGGTAATAGCGATGACTAATACGTAGATGTACTGCCAAGTAGGAAAGTCC78410.16607706941856293No Hit
CTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCT77970.165145123103754No Hit
GTATACATCTGTTCCCAGCATGTTCCTTGAGTCAAATGCAACACTTCAAT77750.16467914994634955No Hit
GTATTGGCCTCTAATGGCCTTAGGTACTAAAGACTGAAATGGTTCAAATT75300.15948990342070896No Hit
GTCATGTACTGGGCATAATGCCAGGCGGGCCATTTACCGTCATTGACGTC74660.15813434514462324No Hit
ATTCTAGACGGGCCGGCCCCTGCGTGTGGCACGGGCGGCCGGGAGGGCGT74020.15677878686853752No Hit
ATCTTATCATGTCTGGATCCGCTGCATTAATGAATCAGGGGATAACGCAG73940.15660934208402683No Hit
TTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGT73290.15523260320987728No Hit
ATTAATAACTAGTCAATAATCAATGTCGACCCAGGTGGCACTTTTCGGGG72150.1528180150305996No Hit
GTGTTATCACTCATGGTTATGGCAGCACTGCATAATTCTCTTACTGTCAT70520.14936557754619384No Hit
GAGTATGAGTATTCAACATTTCCGTGTCGCCCTTATTCCCTTTTTTGCGG69290.1467603639843416No Hit
CTTTTAAAGTTCTGCTATGTGGCGCGGTATTATCCCGTATTGACGCCGGG66550.14095688011484966No Hit
GAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTT65580.13890236210265727No Hit
CCACTACCCTCTCCGTGCTGGAGTACTCATCTACACCCATTTTGCTGATT65120.13792805459172067No Hit
GCATTGTAGGGTTCTGGGACCACTGAATTTTAACAGTTTCCCAGTTTCTG62550.132484640889314No Hit
GAATAAAAGAACTAAGAAATCTAATGTCGCAGTCTCGCACCCGCGAGATA62220.13178568115320732No Hit
CTAGCATACTTACTGACAGCCAGACAGCGACCAAAAGAATTCGGATGGCC62180.13170095876095195No Hit
GCATAATTCTCTTACTGTCATGCCATCCGTAAGATGCTTTTCTGTGACTG61150.12951935716037652No Hit
GGGTAGTGGTGAGCATTGACCGTTTTTTGAGAATCCGGGACCAACGAGGA60660.12848150785524842No Hit
GTGATAACACTGCGGCCAACTTACTTCTGACAACGATCGGAGGACCGAAG60010.12710476898109888No Hit
GATATGAAGAGTTCACAATGGTTGGGAGAAGAGCAACAGCCATACTCAGA59640.12632108685273682No Hit
GGATCAGACCGAGTGATGGTATCACCTCTGGCTGTGACATGGTGGAATAG59480.12598219728371537No Hit
CTACAATGCTATACAATAAAATGGAATTTGAACCATTTCAGTCTTTAGTA59270.12553740472437477No Hit
CTCTTATCTTCAATGTTTGAAGATTGCCCGTAAGCACCTCTTCCTCTCTC59130.125240876351481No Hit
GTAGTACATTTCCTCGTTGGTCCCGGATTCTCAAAAAACGGTCAATGCTC58980.12492316738052342No Hit
CAATTATTGCTTCGGCAATCGACTGTTCGTCTCTCCCACTCACTATCAGC58710.12435129123279977No Hit
GATATATGCAAGGCTGCAATGGGACTGAGAATTAGCTCATCCTTCAGTTT58260.12339816431992699No Hit
GCATTAATGAATCAGGGGATAACGCAGGAAAGAACATGTGAGCAAAAGGC58170.12320753893735245No Hit
GAATTATGCAGTGCTGCCATAACCATGAGTGATAACACTGCGGCCAACTT57930.12269920458382032No Hit
GCATATATCCACGGCTTGCTCTTCTGTTGGGTTCTGCCTAAGGATGTCTA57080.12089885374839397No Hit
ATGTACTACTGTCTCCCGAGGAGGTCAGTGAAACACAGGGAACAGAGAAA56840.12039051939486184No Hit
CCTCAGAACAGCGGACTCCACTCCAGCTGTGCCTTCATCTGGGTCTTCAG56590.11986100444326586No Hit
CTATTAATTGTTGCCGGGAAGCTAGAGTAAGTAGTTCGCCAGTTAATAGT56580.11983982384520203No Hit
GTCTAGAATTGACCTGCATGGTACCCGGGCATGCTCGAGCTAGCAGATCT56000.11861134915749935No Hit
CTCCAGGAGGGGAAGTGAGGAATGATGATGTTGATCAAAGCTTGATTATT55620.11780648643107347No Hit
GTATTCAACATTTCCGTGTCGCCCTTATTCCCTTTTTTGCGGCATTTTGC55200.11691690131239221No Hit
GTTATTAATAGTAATCAATTACGGGGTCATTAGTTCATAGCCCATATATG55120.1167474565278815No Hit
CCCCCATGTTGTGCAAAAAAGCGGTTAGCTCCTTCGGTCCTCCGATCGTT54780.11602731619371097No Hit
TTTTTAAACTATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCG54570.11558252363437035No Hit
CTCCAACATGTATGCAACCATCAAAGGAGAAATTTTGCAATCCTGGAGTT54370.11515891167309357No Hit
CTCCTGGAGTATACATCTGTTCCCAGCATGTTCCTTGAGTCAAATGCAAC53260.11280786528800743No Hit
TAACAAATACAGTTCATTATCCAAAAATCTACAAAACTTATTTTGAAAGA52630.11147348760998554No Hit
CTTATCTTCAATGTTTGAAGATTGCCCGTAAGCACCTCTTCCTCTCTCTT52380.11094397265838957No Hit
GTATTGACCAACACTGATTCAGGACCATTAATCTCCCACATCATTGACGA51970.11007556813777217No Hit
CTTTAAAAGTGCTCATCATTGGAAAACGTTCTTCGGGGCGAAAACTCTCA51550.10918598301909092No Hit
CTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCT51380.10882591285200566No Hit
GTTCATTGATGCTTAGTGCTGGCCCATATCTCTTGTCTTCTTTGCCCAGA51330.10872000986168646No Hit
CTATTGCAATGAAAATAAATTTCCTTTATTAGCCAGAAGTCAGATGCTCA51320.1086988292636226No Hit
TCAATGAACTGAGCAACCTTGCGAAAGGAGAGAAGGCTAATGTGCTAATT51270.10859292627330341No Hit
ATTGAATCCTATGCATCAACTTTTAAGACATTTTCAGAAGGATGCGAAAG51180.10840230089072887No Hit
GAATGAGGAATCCCCTCAGAACAGCGGACTCCACTCCAGCTGTGCCTTCA50920.10785160534106904No Hit
GGCCAATACAGTGGGTTTGTAAGAACTCTGTTCCAACAAATGAGGGATGT50440.10683493663400478No Hit
TAATAAAACTTCTTCCCTTCGCAGCCGCTCCACCAAAGCAAAGTAGAATG50190.1063054216824088No Hit
GTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCT50070.10605125450564273No Hit
GTATATGAAACAGCCCCCTGCTGTCCATTCCTTATTCCATAGAAAAGCCT49920.10573354553468513No Hit
GGACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTC49910.1057123649366213No Hit
GTACTACTGTCTCCCGAGGAGGTCAGTGAAACACAGGGAACAGAGAAACT49730.10533111417147219No Hit
GTATGCTAGAGTCCCGTTTCCGTTTCATTACCAACACCACGTCTCCTTGC49400.1046321544353655No Hit
GGCTAAAGCATGGAACCTTTGGCCCTGTCCATTTTAGAAACCAAGTCAAA49370.104568612641174No Hit
CTCCAGCACGGAGAGGGTAGTGGTGAGCATTGACCGTTTTTTGAGAATCC49180.10416618127796105No Hit
GTTCTGAGGGGATTCCTCATTCTGGGCAAAGAAGACAAGAGATATGGGCC48830.10342486034572665No Hit
GTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAGT48640.10302242898251371No Hit
TTATAATGGTTACAAATAAAGCAATAGCATCACAAATTTCACAAATAAAG47920.10149742592191731No Hit
CAATAATTGTGGCCATGGTATTTTCACAAGAGGATTGTATGATAAAAGCA47630.10088318857806597No Hit
CTAATGGCCTTAGGTACTAAAGACTGAAATGGTTCAAATTCCATTTTATT47600.10081964678387445No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCGTAG151.2150908E-4145.45328145
AGGTGTG1050.0110.82154145
CGTCACG203.819241E-4109.089966145
AATCGCG553.6379788E-12105.7842145
TCCGGTG900.0105.0496145
GGGATAG900.096.968864145
CATAGCG750.096.96886145
CCGACGG750.096.96886145
GAGAACG1050.096.96885145
GCATACG1000.094.54463145
TCCCTTG406.00834E-790.9083145
TCGCAGG651.4551915E-1189.50972145
GTCTCTG1300.089.50972145
ATTATCG651.4551915E-1189.50972145
CCGTTCG1300.089.50972145
GCTTGCG900.088.88812145
GATCTTG1650.088.1535145
GTAACGG502.2218956E-887.271965145
GTTGAAG1100.085.94967145
GATCGGG608.094503E-1084.84775145
Produced by FastQC (version 0.11.5)