FastQCFastQC Report
Sat 5 Dec 2020
H7KKVBGXH_n02_S6_R2_H03_FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7KKVBGXH_n02_S6_R2_H03_FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4608426
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCTTATACTGGAGATCCTCCATACAGCCATGGAACAGGAACAGGATACA1428333.0993879472080055No Hit
AGTAGAAACAAGGCATTTTTTCATGAAGGACAAGTTAAATTCATTATTTT735161.5952518278475123No Hit
GATCTTCATGATCTCAGAGAACTCTTCTTTCTTGATCCGTCCAGACTCGA670631.4552257104703428No Hit
TTCTTGATCCGTCCAGACTCGAAGTCGACCCTGGCATCAATCCGGGCCCT420700.9128930354962844No Hit
GTTAAATTCATTATTTTTGCCGTCTGAGTTCTTCAATGGTGGAACAGATC413980.8983110502371092No Hit
TTTTTAAACAATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCG380840.8263992955512359No Hit
GATCTTATGATCTCAGAGAACTCTTCTTTCTTGATCCGTCCAGACTCGAA373150.8097124701579238No Hit
ATACTGGAGATCCTCCATACAGCCATGGAACAGGAACAGGATACACCATG364990.7920057737717824No Hit
GAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCACTCGCGAGATA309310.6711836101957588No Hit
AATATATTCAATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCA287690.6242695445256146No Hit
AAACAAGGCATTTTTTCATGAAGGACAAGTTAAATTCATTATTTTTGCCG272320.5909175931218165No Hit
CTTCAATGGTGGAACAGATCTTCATGATCTCAGAGAACTCTTCTTTCTTG252440.547779220063423No Hit
CACTAAGACCACTGTGGACCATATGGCCATAATCAAAAAGTACACATCAG250380.5433091472012352No Hit
GTCCAGACTCGAAGTCGACCCTGGCATCAATCCGGGCCCTAGACACCATG248610.5394683564410061No Hit
CCATACAGCCATGGAACAGGAACAGGATACACCATGGACACAGTAAACAG242570.5263619292140093No Hit
GGTCAAATATATTCAATATGGAGAGAATAAAAGAACTGAGAGATCTAATG217120.4711369999214482No Hit
GTAGAAACAAGGTCGTTTTTAAACAATTCGACACTAATTGATGGCCATCC215130.4668188227390436No Hit
CTTCTTTCTTGATCCGTCCAGACTCGAAGTCGACCCTGGCATCAATCCGG212320.4607212961648945No Hit
GTATGCTAGAGTCCCGTTTTCGTTTCATTACCAACACTACGTCCCCTTGC212030.46009201406293604No Hit
CCACATTCCCTTATACTGGAGATCCTCCATACAGCCATGGAACAGGAACA189770.411789187891918No Hit
GTAGAAACAAGGCATTTTTTCATGAAGGACAAGTTAAATTCATTATTTTT185030.4015036804323212No Hit
ATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCT179030.38848405073662895No Hit
GGACAAGTTAAATTCATTATTTTTGCCGTCTGAGTTCTTCAATGGTGGAA178160.38659620443075354No Hit
AAACAAGGTCGTTTTTAAACAATTCGACACTAATTGATGGCCATCCGAAT175250.3802816840283429No Hit
CCTCCATACAGCCATGGAACAGGAACAGGATACACCATGGACACAGTAAA173970.37750416302659523No Hit
ATCTAATGTCGCAGTCCCGCACTCGCGAGATACTCACTAAGACCACTGTG166850.36205420245437375No Hit
ATTCAATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCC163450.35467641229348157No Hit
ACTTAGTTCTTGGAAAGGATGCAGGTGCATTGACTGAAGATCCAGATGAA154470.3351903665155956No Hit
GGATACACCATGGACACAGTAAACAGACACATTCCTGGATTCCCAAGAGG154010.3341921949055925No Hit
AGTTAAATTCATTATTTTTGCCGTCTGAGTTCTTCAATGGTGGAACAGAT150420.3264021164710033No Hit
AATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAGTAAGT149750.32494825782165104No Hit
TCATATAGGAGACCGGTTGGAATTTCTAGCATGGTGGAGGCCATGGTGTC148580.32240943003099104No Hit
GTTCATTGATGCTTAATGCTGGGCCATATCTCTTGTCTTCTTTGCCCAAA139250.30216390585418973No Hit
CCATTTGAATGGATGTCAATCCGACTCTACTTTTCCTAAAAATTCCAGCG138520.3005798509078805No Hit
AGCAGGTACTGATCCAAAATGGAAGACTTTGTGCGACAATGCTTCAATCC138170.2998203725089651No Hit
TTTTTGGACAGTATGGATAGCAAATAGTAGCATTGCCACAACTACTTCAG137170.29765043422634974No Hit
CCATGGACACAGTAAACAGACACATTCCTGGATTCCCAAGAGGAATCGTT135990.29508990705286364No Hit
CTACGTCCCCTTGCCCAATTAGCACATTAGCCTTCTCTCCTTTTGCAAGA130020.2821353755056499No Hit
CTTATGATCTCAGAGAACTCTTCTTTCTTGATCCGTCCAGACTCGAAGTC129910.28189668229456216No Hit
CAATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCA129540.2810938051299945No Hit
GCTTAATGCTGGGCCATATCTCTTGTCTTCTTTGCCCAAAATGAGAAATC128460.2787502717847699No Hit
GAATGAAGTGGATGATGGCAATGAGATACCCAATTACAGCAGACAAGAGA126450.274388695836713No Hit
ACTTACAGTTCTTGGAAAGGATGCAGGTGCATTGACTGAAGATCCAGATG125160.2715894754521392No Hit
CCATATGGCCATAATCAAAAAGTACACATCAGGAAGGCAAGAGAAGAACC120270.26097847725015005No Hit
CACTACGTCCCCTTGCCCAATTAGCACATTAGCCTTCTCTCCTTTTGCAA117480.25492434944165315No Hit
CCCCAGATGTGCCTTCATCTGGATCTTCAGTCAATGCACCTGCATCCTTT115370.2503457796653348No Hit
AAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCTACTTTTCCTA109310.23719595367268564No Hit
CCTATATGAACTGCTAGGGAAAAATTTCTCGAATACTTCTGGTACATCTG108070.2345052302022426No Hit
ACTGTGGACCATATGGCCATAATCAAAAAGTACACATCAGGAAGGCAAGA105560.22905868511287802No Hit
TCTCAGGACAGCAGACTCCACCCCAGATGTGCCTTCATCTGGATCTTCAG105410.2287331943704857No Hit
AGTAGAAACAAGGTCGTTTTTAAACAATTCGACACTAATTGATGGCCATC102440.22228847767111806No Hit
GTATGGATAGCAAATAGTAGCATTGCCACAACTACTTCAGTGCATGTGTG101930.22118180914698424No Hit
GGATACACCATGGATTCGAGAAATTTTTCCCTAGCAGTTCATATAGGAGA101800.22089971717024426No Hit
CCATTTGAATGGATGTCAATCCGACTCTACTTTTCCTAAGCACCACATTC97490.21154728317217203No Hit
GAACAGATCTTCATGATCTCAGAGAACTCTTCTTTCTTGATCCGTCCAGA97480.21152558378934586No Hit
GGCCTCCACCATGCTAGAAATTCCAACCGGTCTCCTATATGAACTGCTAG97370.21128689057825817No Hit
GTGGAACAGATCTTCATGATCTCAGAGAACTCTTCTTTCTTGATCCGTCC96560.20952924056933972No Hit
GGATACACCATGGACACAGTAAACAGAACACACCAATAGGAATCGTTCTA96110.2085527683421628No Hit
TAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCACTCGCGAGATACTC94420.20488557264454285No Hit
ATCTCAGAGAACTCTTCTTTCTTGATCCGTCCAGACTCGAAGTCGACCCT91720.19902673928148137No Hit
CTGTGGACCATATGGCCATAATCAAAAAGTACACATCAGGAAGGCAAGAG91720.19902673928148137No Hit
CCATACAGCCATGGAACAGGAACAGGATACACCATGGATTCGAGAAATTT90660.19672660470190906No Hit
AATTAGCACATTAGCCTTCTCTCCTTTTGCAAGATTGCTCAGTTCATTGA88870.19284241517602754No Hit
GTCCCGCACTCGCGAGATACTCACTAAGACCACTGTGGACCATATGGCCA88780.1926471207305922No Hit
GTCAGTAAGTATGCTAGAGTCCCGTTTTCGTTTCATTACCAACACTACGT86900.1885676367592753No Hit
CTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAGTAA86770.18828554478253529No Hit
TTACCAACACTACGTCCCCTTGCCCAATTAGCACATTAGCCTTCTCTCCT86650.18802515218862145No Hit
ACACTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAG84170.18264370524773535No Hit
GCATTTTTTCATGAAGGACAAGTTAAATTCATTATTTTTGCCGTCTGAGT83660.1815370367236015No Hit
TGGTGGAACAGATCTTCATGATCTCAGAGAACTCTTCTTTCTTGATCCGT83440.18105965030142612No Hit
CTAAGACCACTGTGGACCATATGGCCATAATCAAAAAGTACACATCAGGA83320.18079925770751226No Hit
ATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCACT80640.1749838231101031No Hit
CTCTTCTTTCTTGATCCGTCCAGACTCGAAGTCGACCCTGGCATCAATCC80460.1745932342192323No Hit
ATCCGGGCCCTAGACACCATGGCCTCCACCATGCTAGAAATTCCAACCGG78800.17099113667009083No Hit
CATTAGCCTTCTCTCCTTTTGCAAGATTGCTCAGTTCATTGATGCTTAAT78730.17083924099030776No Hit
CATCAGGAAGGCAAGAGAAGAACCCCGCACTCAGAATGAAGTGGATGATG78120.1695155786379124No Hit
ATCAAAAAGTACACATCAGGAAGGCAAGAGAAGAACCCCGCACTCAGAAT78030.169320284192477No Hit
GAGTTCTTCAATGGTGGAACAGATCTTCATGATCTCAGAGAACTCTTCTT77680.16856080579356164No Hit
ACATTCCCTTATACTGGAGATCCTCCATACAGCCATGGAACAGGAACAGG75770.16441622367376627No Hit
CCTAAGCACCACATTCCCTTATACTGGAGATCCTCCATACAGCCATGGAA75610.16406903354854782No Hit
TCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAGTAAGTATGCTAGAGTCC75490.16380864095463396No Hit
GAACTAAGTCGTTTGGTTGCCTTGTTGTAATTGAATACTGGAGAATTGCC73630.1597725557489694No Hit
TCTTCATGATCTCAGAGAACTCTTCTTTCTTGATCCGTCCAGACTCGAAG73030.15847059277940015No Hit
GATCTAATGTCGCAGTCCCGCACTCGCGAGATACTCACTAAGACCACTGT72340.15697333536439556No Hit
ATGAAGGACAAGTTAAATTCATTATTTTTGCCGTCTGAGTTCTTCAATGG71970.1561704581998279No Hit
ATATTCAATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCAGTC71820.15584496745743556No Hit
AATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCAC71730.1556496730120002No Hit
CCATTTGAATGGATGTCAATCCGACTCTACTTTTCCTGCGCAAAATGCCA71220.15454300448786634No Hit
CCCTTGCCCAATTAGCACATTAGCCTTCTCTCCTTTTGCAAGATTGCTCA69740.15133149582959562No Hit
CCTATATGAACTGCTAGGGAAAAATTTCTCGAATAGATTGCAGCACTTCT69170.15009463100850484No Hit
GGACAGCAGACTCCACCCCAGATGTGCCTTCATCTGGATCTTCAGTCAAT67090.1455811593806649No Hit
CCTCCATACAGCCATGGAACAGGAACAGGATACACCATGGATTCGAGAAA66320.14391030690305107No Hit
GAGAAGAACCCCGCACTCAGAATGAAGTGGATGATGGCAATGAGATACCC65790.14276023961326492No Hit
ATCTTCATGATCTCAGAGAACTCTTCTTTCTTGATCCGTCCAGACTCGAA65780.14273854023043878No Hit
ATTTTTGCCGTCTGAGTTCTTCAATGGTGGAACAGATCTTCATGATCTCA65320.1417403686204357No Hit
AAGCACCACATTCCCTTATACTGGAGATCCTCCATACAGCCATGGAACAG65040.14113278590130338No Hit
GCCATAAGCACCACATTCCCTTATACTGGAGATCCTCCATACAGCCATGG64860.14074219701043264No Hit
GACCACTGTGGACCATATGGCCATAATCAAAAAGTACACATCAGGAAGGC64750.14050350379934493No Hit
GTTCTATTCTCAACACAAGCCAAAGGGGAATTCTTGAGGATGAACAGATG64380.13970062663477725No Hit
GCCATGGAACAGGAACAGGATACACCATGGACACAGTAAACAGAACACAC64310.13954873095499418No Hit
ATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAGTAAGTA64000.1388760500873834No Hit
ATACTCACTAAGACCACTGTGGACCATATGGCCATAATCAAAAAGTACAC63780.13839866366520803No Hit
CTGGAGAATTGCCTCTTACCAGTATCCTCAACCCTGATCCTCTCACATTC62540.13570794019476498No Hit
CTTCATGATCTCAGAGAACTCTTCTTTCTTGATCCGTCCAGACTCGAAGT62520.13566454142911266No Hit
GACCAGAACAGAGTAGGATGCAATTTTCCTCATTGACTGTGAATGTGAGA62010.13455787290497884No Hit
GCACCACATTCCCTTATACTGGAGATCCTCCATACAGCCATGGAACAGGA61130.1326483272162773No Hit
CTTTTGGTCGCTGTCTGGCTGTCAGTAAGTATGCTAGAGTCCCGTTTTCG60620.1315416586921435No Hit
GTGGAGGCCATGGTGTCTAGGGCCCGGATTGATGCCAGGGTCGACTTCGA60360.1309774747386635No Hit
GCCATGGAACAGGAACAGGATACACCATGGACACAGTAAACAGACACATT60240.13071708214474964No Hit
GAAGTGGATGATGGCAATGAGATACCCAATTACAGCAGACAAGAGAATAA59420.12893773275300505No Hit
GTCTGGCTGTCAGTAAGTATGCTAGAGTCCCGTTTTCGTTTCATTACCAA58540.12702818706430352No Hit
ATTCCCCAGTATTCAATTACAACAAGGCAACCAAACGACTTACAGTTCTT58510.12696308891582506No Hit
GAACTCTTCTTTCTTGATCCGTCCAGACTCGAAGTCGACCCTGGCATCAA57850.1255309296492989No Hit
GACACATTCCTGGATTCCCAAGAGGAATCGTTCTATTCTCAACACAAGCC56870.12340439013233587No Hit
GAACAGGAACAGGATACACCATGGACACAGTAAACAGACACATTCCTGGA56750.12314399753842202No Hit
GAACTGAGAGATCTAATGTCGCAGTCCCGCACTCGCGAGATACTCACTAA56390.12236281975668048No Hit
ATCTTATGATCTCAGAGAACTCTTCTTTCTTGATCCGTCCAGACTCGAAG55800.1210825561699374No Hit
ATGCTGGGCCATATCTCTTGTCTTCTTTGCCCAAAATGAGAAATCCTCTC55760.1209957586386328No Hit
GGCAAGAGAAGAACCCCGCACTCAGAATGAAGTGGATGATGGCAATGAGA55620.12069196727906666No Hit
GTTCTTCAATGGTGGAACAGATCTTCATGATCTCAGAGAACTCTTCTTTC55320.12004098579428205No Hit
ATATAGGAGACCGGTTGGAATTTCTAGCATGGTGGAGGCCATGGTGTCTA54990.11932490616101898No Hit
CTGTCTGGCTGTCAGTAAGTATGCTAGAGTCCCGTTTTCGTTTCATTACC54850.11902111480145282No Hit
ATGGAAGACTTTGTGCGACAATGCTTCAATCCAATGATCGTCGAGCTTGC54570.11841353208232053No Hit
GGATACACCATGGACACAGTAAACAGAACACACCAATACTCAGAAAAGGG54460.11817483887123283No Hit
TACCAACACTACGTCCCCTTGCCCAATTAGCACATTAGCCTTCTCTCCTT53770.11667758145622822No Hit
CCATGGCCTCCACCATGCTAGAAATTCCAACCGGTCTCCTATATGAACTG53200.11544071663513746No Hit
GCCCTAGACACCATGGCCTCCACCATGCTAGAAATTCCAACCGGTCTCCT51980.11279339193034671No Hit
CAGAGAACTCTTCTTTCTTGATCCGTCCAGACTCGAAGTCGACCCTGGCA51950.11272829378186826No Hit
ACCCTGGCATCAATCCGGGCCCTAGACACCATGGCCTCCACCATGCTAGA51690.11216410982838826No Hit
TCTGAGTTCTTCAATGGTGGAACAGATCTTCATGATCTCAGAGAACTCTT51480.11170842278903903No Hit
GAACTGTAAGTCGTTTGGTTGCCTTGTTGTAATTGAATACTGGAGAATTG51400.11153482772642981No Hit
ATGTCAATCCGACTCTACTTTTCCTAAAAATTCCAGCGCAAAATGCCATA50980.11062345364773135No Hit
GGAGATCCTCCATACAGCCATGGAACAGGAACAGGATACACCATGGACAC50450.10947338635794522No Hit
GACTCGAAGTCGACCCTGGCATCAATCCGGGCCCTAGACACCATGGCCTC49990.10847521474794215No Hit
GTACACATCAGGAAGGCAAGAGAAGAACCCCGCACTCAGAATGAAGTGGA49770.10799782832576676No Hit
GTGGACCATATGGCCATAATCAAAAAGTACACATCAGGAAGGCAAGAGAA49030.10639207399663138No Hit
GAAACAAGGCATTTTTTCATGAAGGACAAGTTAAATTCATTATTTTTGCC48700.10567599436336832No Hit
ATATGGCCATAATCAAAAAGTACACATCAGGAAGGCAAGAGAAGAACCCC48470.10517690855836678No Hit
CTCCACCCCAGATGTGCCTTCATCTGGATCTTCAGTCAATGCACCTGCAT47820.10376644867466679No Hit
GCCATGGAACAGGAACAGGATACACCATGGATTCGAGAAATTTTTCCCTA47070.10213899496270527No Hit
GTTCCATAATCACATCCTGTGCCTCCTTGGCACTGAGATCTGCATGGCCA46330.10053324063356991No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATAGCG253.6476195E-8145.43561145
CGTATCG450.0129.2761145
CCGCACG2850.0125.0236145
TGACGCG1650.0123.3999145
AGTAGAA115100.0122.203151
CTGACGG1000.0116.34849145
TCGCCGG750.0116.34848145
AGATCGG1400.0114.27083145
CGCTGCG1150.0113.81917145
GCAGGTA20550.0109.691382
ATGTACG5250.0109.42298145
TTGCTCG2650.0107.01865145
CGGGCCG2600.0106.27986145
CGAGTCG553.6379788E-12105.77135145
CAGGTAC22150.0102.071813
GGTCAAA35300.0101.668521
TTCTCGG3950.0101.25264145
AGCGAAA10750.099.818091
CCGCACA4850.097.45685145
CTGTTCG2850.096.95708145
Produced by FastQC (version 0.11.5)