FastQCFastQC Report
Sat 5 Dec 2020
H7KKVBGXH_n02_S6_R2_H02_FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7KKVBGXH_n02_S6_R2_H02_FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2961033
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGTATGAGTTACTAATTTCCTGGACATTATCAGCCATTAGCTGGTTTCC66760.2254618573990901No Hit
GCATTAAGATGTATGAGGTGTATTCCTTCCCCATCTTGGGAATTCTAGTT58450.1973973272165491No Hit
CTATTCCACCGTGCTAACAGTCCCTTTGAGAACTCAGCCATCCATTACAC47050.1588972497098141No Hit
TAATAACACTCCATCAGTAGTTCTTTTGTCAAATGCATATGAGAAAAGGC43440.14670555849934805No Hit
CCCTAATACTCATGCTGTCTTCAACCAAACTTCCAAAATTTTAACAAAGG39610.13377088333699758No Hit
TCTTAGAGCTATTGATGTGTTTTTTCATCTAATTTGGAAAATTTTAGCAA39150.132217371437603No Hit
GTATTTACATGTGTAATCACCTTCCAGATCATGATATAGAATACTTAAGT36410.12296384403686145No Hit
CTTTAGTGATCTGTGGAATAACATCAAGAGACCTATCATTTGGTATAACT36210.12228840408060296No Hit
GGTTATGTATTTGGATGTATTTTATAAAGAAGCCCCTAAATTCCGTATGG33530.11323750866673893No Hit
GTCTAGTTAATAAAGCCCTACTATTCCACCGTGCTAACAGTCCCTTTGAG32760.11063706483514367No Hit
GTACAGTGGTTATGTATTTGGATGTATTTTATAAAGAAGCCCCTAAATTC32560.10996162487888518No Hit
ACCTAAGACATAATAACACTCCATCAGTAGTTCTTTTGTCAAATGCATAT32350.10925241292481375No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGACGCG1350.075.408516145
CGTATCG300.001913470772.715355145
GATCGCG502.2670556E-672.71535145
AGCGAAA6300.070.208211
TCGTTCG754.7457434E-967.86766145
GCGAAAG9000.049.941292
CGAAAGC9250.048.578393
TCGCATG1609.822543E-1040.902386145
AGATTCG800.002007813736.357677145
CTATGCG850.002748703134.1028184
GCATTAA27250.033.793711
GTAGAAA35000.032.9403151
AGATGTA29950.030.9720277
CGCATAG2202.202978E-829.747189145
GTCTAGT21300.029.2763881
GCTATCG1255.1688787E-428.9878858
TCGCCGG1306.3744595E-427.967443145
TAAGATG41000.027.5724435
CATTAAG35050.026.6816232
GGAATCG1100.00953572626.441946145
Produced by FastQC (version 0.11.5)