FastQCFastQC Report
Sat 5 Dec 2020
H7KKVBGXH_n02_S6_R2_H01_FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7KKVBGXH_n02_S6_R2_H01_FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2581222
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGTATGAGTTACTAATTTCCTGGACATTATCAGCCATTAGCTGGTTTCC34540.13381258954092287No Hit
CCCTTATACTGGAGATCCTCCATACAGCCATGGAACAGGAACAGGATACA26970.1044853949021045No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATCG702.910383E-1183.12308145
GGTCGCG1400.083.12308145
GATCGCG1251.8189894E-1258.186157145
AGCGAAA5150.056.3249131
CGATCGG1551.4551915E-1146.924316145
TGACGCG1053.743111E-641.56154145
CAATTCG5600.041.4093329
GCGAAAG7950.037.3993871
CGAAAGC8500.034.96792
GTAGAAA31300.033.5947531
AGTAGAA31050.029.4277271
GCATTAA21150.028.1158521
AGTACTG24250.026.8942015
TATACCG1358.1090775E-426.8388635
CCGACGG1906.9850994E-626.796255145
CATTAAG23450.025.3498632
GGGCGAT4400.024.7039557
CTTATAT10900.024.60822
TTAGTAC14950.024.2376423
GTATACC9950.024.0331294
Produced by FastQC (version 0.11.5)