FastQCFastQC Report
Sat 5 Dec 2020
H7KKVBGXH_n02_S6_R2_G02_FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7KKVBGXH_n02_S6_R2_G02_FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3295680
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACATAGCACCGTGTTCAACCTTGAGAATTTCTGGTAACAACTCATGGGCT33470.10155719001844839No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGAAA7750.049.5916751
TGACGCG1255.0640665E-946.54177145
CGTATCG750.001461838.78481145
GGTCGCG951.0110779E-438.27448145
GCGAAAG10700.037.2627642
CGAAAGC11100.035.2588423
ATACACG2455.2750693E-1132.65048145
CGACGAG2252.7388523E-829.088608145
TATATCG1000.00598655429.088608145
AGTACTG23050.028.925965
TAAGGCG2559.2235496E-825.66642145
ACGATAG1701.01082E-425.666418145
TAGTACT31550.025.4969314
GTCTAGT10500.024.172041
GAGCGAA6050.023.9722711
GCACCGT25700.023.1244496
CCGACGG2603.1240415E-622.37585145
GTACGTT3955.9299055E-1022.0178456
GTACTGT24700.022.0067046
GTAGAAA24250.021.8295971
Produced by FastQC (version 0.11.5)