FastQCFastQC Report
Sat 5 Dec 2020
H7KKVBGXH_n02_S6_R2_G01_FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7KKVBGXH_n02_S6_R2_G01_FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4100242
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCTTATACTGGAGATCCTCCATACAGCCATGGAACAGGAACAGGATACA319660.7796125204317208No Hit
TTTTTAAACAATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCG298720.7285423640848516No Hit
GAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCACTCGCGAGATA264290.6445717106453717No Hit
CACTAAGACCACTGTGGACCATATGGCCATAATCAAAAAGTACACATCAG228730.5578451223122928No Hit
AATATATTCAATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCA210640.5137257752103412No Hit
TTTTTGGACAGTATGGATAGCAAATAGTAGCATTGCCACAACTACTTCAG195940.4778742327891866No Hit
GTTCATTGATGCTTAATGCTGGGCCATCTCTTGTCTTCTTTGCCCAAAAT194640.47470368822132936No Hit
AGCAGGTACTGATCCAAAATGGAAGACTTTGTGCGACAATGCTTCAATCC185830.4532171515730047No Hit
GGTCAAATATATTCAATATGGAGAGAATAAAAGAACTGAGAGATCTAATG177470.43282811112124603No Hit
CCTATATGAACTGCTAGGGAAAAATTTCTCGAATAGATTGCAGCACTTCT172660.4210970962201743No Hit
AGTAGAAACAAGGCATTTTTTCATGAAGGACAAGTTAAATTCATTATTTT166420.4058784822944597No Hit
GTAGAAACAAGGTCGTTTTTAAACAATTCGACACTAATTGATGGCCATCC159100.38802587749698675No Hit
GTATGCTAGAGTCCCGTTTTCGTTTCATTACCAACACTACGTCCCCTTGC152620.37222193226643696No Hit
GTATGGATAGCAAATAGTAGCATTGCCACAACTACTTCAGTGCATGTGTG143840.3508085620312167No Hit
GAATGAAGTGGATGATGGCAATGAGACCCAATTACAGCAGACAAGAGAAT141980.3462722444187441No Hit
ATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCT138540.3378824957161065No Hit
GCTTAATGCTGGGCCATCTCTTGTCTTCTTTGCCCAAAATGAGAAATCCT138230.3371264427806944No Hit
CCCCAGATGTGCCTTCATCTGGATCTTCAGTCAATGCACCTGCATCCTTT134190.3272733658159689No Hit
AAACAAGGTCGTTTTTAAACAATTCGACACTAATTGATGGCCATCCGAAT133840.3264197576630843No Hit
ATCTAATGTCGCAGTCCCGCACTCGCGAGATACTCACTAAGACCACTGTG133750.3262002584237711No Hit
ATTCAATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCC132820.3239320996175348No Hit
CCATATGGCCATAATCAAAAAGTACACATCAGGAAGGCAAGAGAAGAACC129310.3153716292843203No Hit
TCTCAGGACAGCAGACTCCACCCCAGATGTGCCTTCATCTGGATCTTCAG125600.30632338286374317No Hit
TTCTTGATCCGTCCAGACTCGAAGTCGACCCTGGCATCAATCCGGGCCCT124150.3027870062303639No Hit
ACTGTGGACCATATGGCCATAATCAAAAAGTACACATCAGGAAGGCAAGA111710.2724473335964072No Hit
GTTAAATTCATTATTTTTGCCGTCTGAGTTCTTCAATGGTGGAACAGATC109800.26778907196209395No Hit
AATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAGTAAGT104900.25583855782170906No Hit
ACTTTTCCTAAAAATTCCAGCGCAAAATGCCATAAGCACCACATTCCCTT98200.23949805889506032No Hit
CTGTGGACCATATGGCCATAATCAAAAAGTACACATCAGGAAGGCAAGAG95180.23213263997588435No Hit
CTTCAATGGTGGAACAGATCTTCATGATCTCAGAGAACTCTTCTTTCTTG95150.23205947356277995No Hit
GTCTATATGCGTCTCCACATGGGGAAGATCCGAAAATCGAAACTAACAAG90000.21949923931319174No Hit
CATCAGGAAGGCAAGAGAAGAACCCCGCACTCAGAATGAAGTGGATGATG89930.21932851768261485No Hit
GAATAATGGACATGATTCCAGAGAGGAATGAACAAGGACAAACCCTCTGG89500.21827979909478512No Hit
CAATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCA89140.21740180213753235No Hit
ATGCTGGGCCATCTCTTGTCTTCTTTGCCCAAAATGAGAAATCCTCTCAG87380.21310937256874105No Hit
CTACGTCCCCTTGCCCAATTAGCACATTAGCCTTCTCTCCTTTTGCAAGA84210.2053781215840431No Hit
CACTACGTCCCCTTGCCCAATTAGCACATTAGCCTTCTCTCCTTTTGCAA82210.2005003607104166No Hit
GGATACACCATGGACACAGTAAACAGAACACACCAATACTCAGAAAAGGG82120.20028086147110344No Hit
ATCAAAAAGTACACATCAGGAAGGCAAGAGAAGAACCCCGCACTCAGAAT82110.20025647266673527No Hit
CTACTTTTCCTAAAAATTCCAGCGCAAAATGCCATAAGCACCACATTCCC81680.1992077540789056No Hit
ATACTGGAGATCCTCCATACAGCCATGGAACAGGAACAGGATACACCATG81130.19786636983865832No Hit
CATTAGCCTTCTCTCCTTTTGCAAGATTGCTCAGTTCATTGATGCTTAAT79830.1946958252708011No Hit
CAAGAGAATAATGGACATGATTCCAGAGAGGAATGAACAAGGACAAACCC79610.19415927157470217No Hit
ATGCACCTGCATCCTTTCCAAGAACTGTAAGTCGTTTGGTTGCCTTGTTG79230.19323249700871314No Hit
CTTCTTTCTTGATCCGTCCAGACTCGAAGTCGACCCTGGCATCAATCCGG79040.19276910972571862No Hit
AGTAGAAACAAGGTCGTTTTTAAACAATTCGACACTAATTGATGGCCATC78010.190257062875801No Hit
GTTGTAATGAGGAAAATTGCATCCTACTCTGTTCTGGTGGGGCAGCAGCA76990.18776940483025148No Hit
CTTGTCTTCTTTGCCCAAAATGAGAAATCCTCTCAGGACAGCAGACTCCA76960.18769623841714708No Hit
CTAAGACCACTGTGGACCATATGGCCATAATCAAAAAGTACACATCAGGA75990.18533052439343825No Hit
GTCCAGACTCGAAGTCGACCCTGGCATCAATCCGGGCCCTAGACACCATG75940.18520858037159757No Hit
TAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCACTCGCGAGATACTC75670.184550082653658No Hit
ATGGAAGACTTTGTGCGACAATGCTTCAATCCAATGATCGTCGAGCTTGC72800.177550495800004No Hit
GTCCCGCACTCGCGAGATACTCACTAAGACCACTGTGGACCATATGGCCA72790.17752610699563587No Hit
CTACTTTTCCAGCGCAAAATGCCATAAGCACCACATTCCCTTATACTGGA72020.17564816905928968No Hit
GTTCTGGTGGGGCAGCAGCAAAGGGGAGAAGTTTTATTATCGTTTGTTTT71680.17481894971077316No Hit
CATTTAGACAATAACTTCTCAATAAAGCAAGATAGAAAATTGCTTCTCAT71120.17345317666615775No Hit
GGACAGCAGACTCCACCCCAGATGTGCCTTCATCTGGATCTTCAGTCAAT70200.17120940666428958No Hit
CACCAATACTCAGAAAAGGGAAAGTGGACGACAAACACAGAGACTGGTGC70190.17118501785992143No Hit
GAATGGATGATGGCAATGAGATACCCAATTACAGCAGACAAGAGAATAAT69240.16886808144494886No Hit
GAGAAGAACCCCGCACTCAGAATGAAGTGGATGATGGCAATGAGACCCAA69090.16850224937942687No Hit
CCATACAGCCATGGAACAGGAACAGGATACACCATGGACACAGTAAACAG68010.16586825850766856No Hit
ATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCACT67820.16540487122467404No Hit
AATTAGCACATTAGCCTTCTCTCCTTTTGCAAGATTGCTCAGTTCATTGA66870.16308793480970146No Hit
ATACTCACTAAGACCACTGTGGACCATATGGCCATAATCAAAAAGTACAC66120.16125877448209153No Hit
ACTCTGTTCTGGTGGGGCAGCAGCAAAGGGGAGAAGTTTTATTATCGTTT65820.16052711035104758No Hit
GACCACTGTGGACCATATGGCCATAATCAAAAAGTACACATCAGGAAGGC65710.16025883350299813No Hit
GAATGGATGATGGCAATGAGACCCAATTACAGCAGACAAGAGAATAATGG64910.15830772915354752No Hit
GTCAGTAAGTATGCTAGAGTCCCGTTTTCGTTTCATTACCAACACTACGT64810.15806384110986618No Hit
GAATATGGGGAAGATCCGAAAATCGAAACTAACAAGTTTGCTGCAATATG64120.15638101360846507No Hit
TTACCAACACTACGTCCCCTTGCCCAATTAGCACATTAGCCTTCTCTCCT63710.15538107262937162No Hit
GTTTTATTATCGTTTGTTTTGCTCCAGAGGGTTTGTCCTTGTTCATTCCT63320.15442990925901445No Hit
AAACAAGGCATTTTTTCATGAAGGACAAGTTAAATTCATTATTTTTGCCG62700.15291780338819028No Hit
GGCAAGAGAAGAACCCCGCACTCAGAATGAAGTGGATGATGGCAATGAGA61790.15069842219069018No Hit
CTCCTATATGAACTGCTAGGGAAAAATTTCTCGAATAGATTGCAGCACTT61720.15052770056011328No Hit
CATATAGACTGTTGAATACAGATTTTGCCAGTAAGGTCCTGCACACTTTC61120.14906437229802533No Hit
CATCTGGATCTTCAGTCAATGCACCTGCATCCTTTCCAAGAACTGTAAGT60890.1485034297975583No Hit
GATCTAATGTCGCAGTCCCGCACTCGCGAGATACTCACTAAGACCACTGT60710.1480644313189319No Hit
ATCCTACTCTGTTCTGGTGGGGCAGCAGCAAAGGGGAGAAGTTTTATTAT60090.1465523254481077No Hit
ATTCCAGAGAGGAATGAACAAGGACAAACCCTCTGGAGCAAAACAAACGA59820.14589382773016812No Hit
CTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAGTAA59810.14586943892579998No Hit
GGCCTCCACCATGCTAGAAATTCCAACCGGTCTCCTATATGAACTGCTAG59570.1452841076209648No Hit
TCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAGTAAGTATGCTAGAGTCC59170.14430855544623952No Hit
GTACACATCAGGAAGGCAAGAGAAGAACCCCGCACTCAGAATGAAGTGGA59150.14425977783750324No Hit
ATATTCAATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCAGTC58190.14191845261816255No Hit
ACACTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAG57790.14094290044343724No Hit
AATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCAC57180.13945518337698115No Hit
TAATAAAACTTCTCCCCTTTGCTGCTGCCCCACCAGAACAGAGTAGGATG55940.13643097163533274No Hit
GAGTAGGATGCAATTTTCCTCATTACAACAAGGCAACCAAACGACTTACA55540.13545541946060746No Hit
CCATTTGAATGGATGTCAATCCGACTCTACTTTTCCTAAAAATTCCAGCG55430.135187142612558No Hit
CAGTAAACAGAACACACCAATACTCAGAAAAGGGAAAGTGGACGACAAAC54070.13187026521849196No Hit
CAATTACAGCAGACAAGAGAATAATGGACATGATTCCAGAGAGGAATGAA53640.13082154663066228No Hit
CATTACAACAAGGCAACCAAACGACTTACAGTTCTTGGAAAGGATGCAGG53470.13040693695640404No Hit
GTCAATGCACCTGCATCCTTTCCAAGAACTGTAAGTCGTTTGGTTGCCTT53470.13040693695640404No Hit
ATAATAAAACTTCTCCCCTTTGCTGCTGCCCCACCAGAACAGAGTAGGAT53330.13006549369525017No Hit
GCTTCATATAGCCCCCCAAGATCGAAGGTTCCAGGTTCCAGGTTGTCCCT53170.12967527282536007No Hit
CCTCCATACAGCCATGGAACAGGAACAGGATACACCATGGACACAGTAAA50410.1229439628197555No Hit
GAACAGAGTAGGATGCAATTTTCCTCATTACAACAAGGCAACCAAACGAC50260.12257813075423353No Hit
CTCCACCCCAGATGTGCCTTCATCTGGATCTTCAGTCAATGCACCTGCAT50260.12257813075423353No Hit
ATATGGCCATAATCAAAAAGTACACATCAGGAAGGCAAGAGAAGAACCCC50020.12199279944939835No Hit
GCCTTCATCTGGATCTTCAGTCAATGCACCTGCATCCTTTCCAAGAACTG49820.12150502336203571No Hit
ATTAGCACATTAGCCTTCTCTCCTTTTGCAAGATTGCTCAGTTCATTGAT49410.12050508238294227No Hit
GATCTTCATGATCTCAGAGAACTCTTCTTTCTTGATCCGTCCAGACTCGA49310.12026119433926095No Hit
GGACAAGTTAAATTCATTATTTTTGCCGTCTGAGTTCTTCAATGGTGGAA49280.12018802792615654No Hit
AACTACTTCAGTGCATGTGTGAGGAAGGAGTTGAACCAAGATGCATTAAG49220.12004169509994776No Hit
CTCTTGTCTTCTTTGCCCAAAATGAGAAATCCTCTCAGGACAGCAGACTC49130.11982219586063457No Hit
CCACATTCCCTTATACTGGAGATCCTCCATACAGCCATGGAACAGGAACA48610.11855397803349169No Hit
TGATTCCAGAGAGGAATGAACAAGGACAAACCCTCTGGAGCAAAACAAAC48030.11713942738014001No Hit
ATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAGTAAGTA47750.11645654085783229No Hit
CCTGCATCCTTTCCAAGAACTGTAAGTCGTTTGGTTGCCTTGTTGTAATG47680.11628581922725537No Hit
GTGGACCATATGGCCATAATCAAAAAGTACACATCAGGAAGGCAAGAGAA47460.11574926553115646No Hit
CTGTAAGTCGTTTGGTTGCCTTGTTGTAATGAGGAAAATTGCATCCTACT46630.11372499476860146No Hit
CACCAGAACAGAGTAGGATGCAATTTTCCTCATTACAACAAGGCAACCAA46530.11348110672492014No Hit
CCCTTGCCCAATTAGCACATTAGCCTTCTCTCCTTTTGCAAGATTGCTCA45550.11109100389684316No Hit
GTCTAAATGTGTGCATATTGCAGCAAACTTGTTAGTTTCGATTTTCGGAT44660.10892040030807937No Hit
GAACTGAGAGATCTAATGTCGCAGTCCCGCACTCGCGAGATACTCACTAA44600.10877406748187059No Hit
GCAAATAGTAGCATTGCCACAACTACTTCAGTGCATGTGTGAGGAAGGAG44530.10860334585129365No Hit
AGATTGCTCAGTTCATTGATGCTTAATGCTGGGCCATCTCTTGTCTTCTT43740.10667663030621119No Hit
GCATTAAGCAAAACCCAGGGATCATTAATCAGGCACTCCTCGATTGCTTC43090.10509135802228259No Hit
GGAATGAACAAGGACAAACCCTCTGGAGCAAAACAAACGATAATAAAACT42600.10389630660824409No Hit
GTATATGTAACACAACAGGGGTAGAGAAGCCTAAATTTCTTCCTGATTTG42520.10370119617329904No Hit
AGTAGAAACAAGGTACTTTTTTGGACAGTATGGATAGCAAATAGTAGCAT42340.10326219769467265No Hit
GCTCAGTTCATTGATGCTTAATGCTGGGCCATCTCTTGTCTTCTTTGCCC41980.1023842007374199No Hit
GTCTGGCTGTCAGTAAGTATGCTAGAGTCCCGTTTTCGTTTCATTACCAA41670.1016281478020078No Hit
CCTATATGAACTGCTAGGGAAAAATTTCTCGAATGTCGTCCACTTTCCCT41500.10121353812774954No Hit
GTAGAAACAAGGCATTTTTTCATGAAGGACAAGTTAAATTCATTATTTTT41310.10075015084475501No Hit
GAAGTGGATGATGGCAATGAGACCCAATTACAGCAGACAAGAGAATAATG41300.10072576204038688No Hit
TCTTCAGTCAATGCACCTGCATCCTTTCCAAGAACTGTAAGTCGTTTGGT41110.10026237475739237No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATCG500.0130.90617145
ACGATAG1650.0123.413246145
GCAGGTA28300.0107.8392262
CAGGTAC29550.0103.013333
AGTAGAA47600.099.92971
GGTACTG32550.093.723385
CGACGAG950.091.864145
AGCGAAA7000.089.083671
CGGATCG259.27364E-487.27079145
TGACGCG850.085.5596145
ACTGATC36500.083.986998
GGTCGCG702.910383E-1183.11504145
AGATCGG2950.081.35413145
CGAGTCG451.2109813E-680.80629145
GTACTGA40650.077.9038546
AACTGCG2450.077.17825145
TACTGAT40150.075.6270457
GACAGTA47300.068.791357
GAGCGAA1900.068.693651
CATTCCG1800.068.68535145
Produced by FastQC (version 0.11.5)