FastQCFastQC Report
Sat 5 Dec 2020
H7KKVBGXH_n02_S6_R2_E03_FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7KKVBGXH_n02_S6_R2_E03_FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1873261
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATAATAAGGATATGTAATGCAACAACATTTTTAGTGAAAACAAAACAT30120.16078912655524244No Hit
GTATTATACATCTGAAACTAATGTAACATTGTGTGTCCACTATCATCAAT29240.15609143627075991No Hit
GTTGAATATATTGTGGACCTCTAGGTCGACAGCCCTGAAGCTATTTTAAA26180.13975628596335482No Hit
TCTTAGAGCTATTGATGTGTTTTTTCATCTAATTTGGAAAATTTTAGCAA25740.13740744082111356No Hit
CAATAGGAGAACCAAAAATATGAAAGAACTGAATATAACTTCTAGGACTA24980.1333503446663332No Hit
GTATAACACAGCTTATCAGAGTTTTTTCTCTGCCCTCATGTGCTTGTTAT22930.12240686161725461No Hit
AAATAATAGGTCCTGGGTAGGGATAAATCTAATGCACCTTTTAAAATAGC20880.11146337856817604No Hit
CCCTACACCTGCTTCAACAGGAGCGTGAAGAAAAGACACACACCAAACTG20710.11055587021776463No Hit
ATGTATAATAAGGATATGTAATGCAACAACATTTTTAGTGAAAACAAAAC20200.10783334516653044No Hit
CTAATACATAGGCTCGTTGTAACTAAACACTTTGAAGATGTTATAAAAGA20000.10676568828369352No Hit
GTAGAGAAGGCTATGTATAATAAGGATATGTAATGCAACAACATTTTTAG19770.10553788286843105No Hit
CTTTAGTGATCTGTGGAATAACATCAAGAGACCTATCATTTGGTATAACT19630.10479052305044519No Hit
GCCCAGAACCAACCCACACACCACCACCTAAACAGGGCGGTTTCAAGCCA19480.10398978038831748No Hit
ACCCAGGACCTATTATTTAGTGGGAAAAATGAAGTGCAAAATAAACGTAT19340.10324242057033163No Hit
CCATAGTGGTGTATGGTGACAGACGGTGGCTCCTTTTTTCCTGAGCACAG18940.10110710680465776No Hit
GTGTTATACGCTACCCACTTGTCTTTACATCGGATAATTAACTCTATTAA18810.10041312983081374No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTTCG300.001912919872.719574145
AGCGAAA3550.067.4323041
GCGAAAG4600.052.027642
CGAAAGC5400.042.964193
AGTTTCG700.001041268941.554043145
GGTCGCG951.0110688E-438.27346145
GTAGAAA9750.035.7124021
CGACGAG2052.8376235E-1035.47296145
TGACGCG850.00270222934.220978145
CTATTGA14400.032.717899
GCATTAA8200.031.8471851
GCTATTG15150.031.576638
ACCGTGC9450.029.9135028
GAGCGAA2303.472087E-828.3855361
TGTAACG1555.390709E-528.149513145
GAGCTAT18100.028.0327366
ACGATCG1306.371411E-427.969069145
CTACGGT1100.00968117226.3594594
CAATAGG15150.026.3349481
TGTCTCG1958.556577E-626.104464145
Produced by FastQC (version 0.11.5)