FastQCFastQC Report
Sat 5 Dec 2020
H7KKVBGXH_n02_S6_R2_E02_FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7KKVBGXH_n02_S6_R2_E02_FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4627073
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTAAACAATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCG141880.3066301309704861No Hit
AATATATTCAATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCA119940.2592135460149429No Hit
GAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCACTCGCGAGATA98620.21313690101712246No Hit
AGCAGGTACTGATCCAAAATGGAAGACTTTGTGCGACAATGCTTCAATCC94580.20440567935712273No Hit
TTTTTGGACAGTATGGATAGCAAATAGTAGCATTGCCACAACTACTTCAG93050.2010990533324199No Hit
GTAGAAACAAGGTCGTTTTTAAACAATTCGACACTAATTGATGGCCATCC81570.17628855217974734No Hit
GGTCAAATATATTCAATATGGAGAGAATAAAAGAACTGAGAGATCTAATG79990.17287386648103456No Hit
CACTAAGACCACTGTGGACCATATGGCCATAATCAAAAAGTACACATCAG69300.1497707081777184No Hit
GTATGGATAGCAAATAGTAGCATTGCCACAACTACTTCAGTGCATGTGTG65800.14220653099702554No Hit
AAACAAGGTCGTTTTTAAACAATTCGACACTAATTGATGGCCATCCGAAT64110.138554114015491No Hit
GTATGCTAGAGTCCCGTTTTCGTTTCATTACCAACACTACGTCCCCTTGC62180.13438301059870894No Hit
CCCTTATACTGGAGATCCTCCATACAGCCATGGAACAGGAACAGGATACA61170.132200205183709No Hit
ATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCT60410.13055769813875856No Hit
ATTCAATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCC58660.12677560954841213No Hit
ATCTAATGTCGCAGTCCCGCACTCGCGAGATACTCACTAAGACCACTGTG50480.10909704688039286No Hit
AATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAGTAAGT47690.10306731707064055No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGAAA13850.065.447881
CAATTCG45250.054.7770589
GCGAAAG17450.052.7620352
GCAGGTA28750.050.179752
CAGGTAC29050.049.153933
GGTACTG30050.048.955925
CGAAAGC19050.047.561243
GTACTGA43500.047.1491976
ACTGATC32700.046.765038
GTAGAAA52700.039.904581
GGTCGTT25350.037.4516077
GACAGTA49250.034.87547
TACTGAT43050.034.8477137
AGGTCGT27450.034.5864566
CAGTATG45600.034.4894379
CGTATCG850.002701852534.223007145
AAGGTCG30000.034.060545
TTATACT46350.032.99284
AGTAGAA56050.032.8619581
TATACTG46950.032.4143525
Produced by FastQC (version 0.11.5)