FastQCFastQC Report
Sat 5 Dec 2020
H7KKVBGXH_n02_S6_R2_E01_FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7KKVBGXH_n02_S6_R2_E01_FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4271784
Sequences flagged as poor quality0
Sequence length151
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATCG406.565915E-572.721016145
CAATTCG11550.051.4504479
AGCGAAA11050.048.5557141
TGACGCG3900.042.886753145
GCGAAAG14050.038.693122
CGAAAGC14450.037.614543
CCGACGG3403.6379788E-1227.805096145
GTAGAAA36550.024.995051
TAGTACT41150.023.772814
GTATACC13200.023.6053854
GCCGTCG1600.00213814422.725319145
AGTACTG33100.022.1105615
TATACCC17200.021.906925
GGTCGTT9800.021.4449027
CTATACG8550.021.2004361
CTAGTAC12500.020.8715573
GTATTAG15350.020.783331
ATACACG4651.9826984E-1020.330606145
TTAGTAC28400.020.1590983
TTTAAAC38150.020.1360193
Produced by FastQC (version 0.11.5)