FastQCFastQC Report
Sat 5 Dec 2020
H7KKVBGXH_n02_S6_R2_D02_FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7KKVBGXH_n02_S6_R2_D02_FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3872436
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGATAAAGTGGACGACAAACACAGAGACTGGTGCACCCCAGCTCAACCCG914542.361665886795805No Hit
CCCTTATACTGGAGATCCTCCATACAGCCATGGAACAGGAACAGGATAAA500751.2931136886445638No Hit
CTTCTGGTACATCTGTTCATCCTCAAGAATTCCCCTTTGGCTTGTGTTGA496961.2833265675662555No Hit
AGTAGAAACAAGGCATTTTTTCATGAAGGACAAGTTAAATTCATTATTTT430211.110954448311089No Hit
CCCTTATACTGGAGATCCTCCATACAGCCATGGAACAGGAACAGGATAGA318190.8216791704239915No Hit
CCTATATGAACTGCTAGGGAAAAATTTCTCGAATAGATTGCAGCACTTCT309800.8000132216516942No Hit
GTTAAATTCATTATTTTTGCCGTCTGAGTTCTTCAATGGTGGAACAGATC267360.690418124405413No Hit
GGATAGAAGTGCTGCAATCTATTCGAGAAATTTTTCCCTAGCAGTTCATA264590.6832650042505545No Hit
TTCTTGATCCGTCCAGACTCGAAGTCGACCCTGGCATCAATCCGGGCCCT260790.6734520596337809No Hit
TTTTTAAACAATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCG212600.5490084277700135No Hit
CCATTTGAATGGATGTCAATCCGACTCTACTTTTCCTAAAAATTCCAGCG202270.5223327125354686No Hit
AATATATTCAATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCA198210.5118483559186No Hit
CTTCAATGGTGGAACAGATCTTCATGATCTCAGAGAACTCTTCTTTCTTG189480.4893044068384862No Hit
GCTAACCGGTCTCCTATATGAACTGCTAGGGAAAAATTTCTCGAATAGAT175890.4542102180642882No Hit
GTCCAGACTCGAAGTCGACCCTGGCATCAATCCGGGCCCTAGACACCATG172060.44431980283211914No Hit
GAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCACTCGCGAGATA170340.4398781542161058No Hit
CCCTTATACTGGAGATCCTCCATACAGCCATGGAACAGGAACAGGATACA161340.4166369695974317No Hit
AAACAAGGCATTTTTTCATGAAGGACAAGTTAAATTCATTATTTTTGCCG157480.406669083749867No Hit
CCACATTCCCTTATACTGGAGATCCTCCATACAGCCATGGAACAGGAACA150340.3882310772857189No Hit
GGTCAAATATATTCAATATGGAGAGAATAAAAGAACTGAGAGATCTAATG136770.3531885355884513No Hit
ATACTGGAGATCCTCCATACAGCCATGGAACAGGAACAGGATAAAGTGGA135780.3506320052803972No Hit
CTCCTATATGAACTGCTAGGGAAAAATTTCTCGAATAGATTGCAGCACTT134260.34670682743368775No Hit
CTTCTTTCTTGATCCGTCCAGACTCGAAGTCGACCCTGGCATCAATCCGG132990.3434272380486082No Hit
GATCTTCATGATCTCAGAGAACTCTTCTTTCTTGATCCGTCCAGACTCGA126470.326590290969302No Hit
GGACAAGTTAAATTCATTATTTTTGCCGTCTGAGTTCTTCAATGGTGGAA125180.32325905450729203No Hit
GTAGAAACAAGGTCGTTTTTAAACAATTCGACACTAATTGATGGCCATCC118890.30701604881268535No Hit
ATGCTAGAAATTAACCGGTCTCCTATATGAACTGCTAGGGAAAAATTTCT108550.2803145100396753No Hit
CACTAAGACCACTGTGGACCATATGGCCATAATCAAAAAGTACACATCAG103280.26670550526851833No Hit
GTAGAAACAAGGCATTTTTTCATGAAGGACAAGTTAAATTCATTATTTTT99600.25720244311332713No Hit
AAACAAGGTCGTTTTTAAACAATTCGACACTAATTGATGGCCATCCGAAT99090.25588544265160224No Hit
GCCATAAGCACCACATTCCCTTATACTGGAGATCCTCCATACAGCCATGG98190.25356132418973487No Hit
CCATACAGCCATGGAACAGGAACAGGATAAAGTGGACGACAAACACAGAG97260.2511597351124718No Hit
ATTCAATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCC95710.24715708665036684No Hit
ATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCT95590.24684720418878453No Hit
AAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCTACTTTTCCTA95540.24671808649645854No Hit
ATGCTAACCGGTCTCCTATATGAACTGCTAGGGAAAAATTTCTCGAATAG93540.24155337880341987No Hit
AGTTAAATTCATTATTTTTGCCGTCTGAGTTCTTCAATGGTGGAACAGAT92000.23757655387978008No Hit
TGCTAACCGGTCTCCTATATGAACTGCTAGGGAAAAATTTCTCGAATAGA90560.2338579643407922No Hit
GGATACACCATGGACACAGTAAACAGAACACACCAATACTCAGAAAAGGG87810.22675649126286399No Hit
ATGTCAATCCGACTCTACTTTTCCTAAAAATTCCAGCGCAAAATGCCATA86610.22365766664704076No Hit
CCTCCATACAGCCATGGAACAGGAACAGGATAAAGTGGACGACAAACACA85210.22004237126191367No Hit
ATCTAATGTCGCAGTCCCGCACTCGCGAGATACTCACTAAGACCACTGTG84060.21707266433841646No Hit
AAGCACCACATTCCCTTATACTGGAGATCCTCCATACAGCCATGGAACAG83740.21624631110753026No Hit
GCATTAGCCTTCTCTCCTTTTGCAAGATTGCTCAGTTCATTGATGCTTAA83110.21461942818422308No Hit
GAACAGATCTTCATGATCTCAGAGAACTCTTCTTTCTTGATCCGTCCAGA80280.2073113667985733No Hit
GTGGAACAGATCTTCATGATCTCAGAGAACTCTTCTTTCTTGATCCGTCC78040.20152689418236996No Hit
ATCTCAGAGAACTCTTCTTTCTTGATCCGTCCAGACTCGAAGTCGACCCT75010.19370236202741634No Hit
ATACTGGAGATCCTCCATACAGCCATGGAACAGGAACAGGATAGAAGTGC74740.1930051264888561No Hit
AATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAGTAAGT72660.18763383048809584No Hit
TTTTTGGACAGTATGGATAGCAAATAGTAGCATTGCCACAACTACTTCAG70940.18319218187208258No Hit
GAATGGATGTCAATCCGACTCTACTTTTCCTAAAAATTCCAGCGCAAAAT67230.17361164910149582No Hit
GGCAAACCATTTGAATGGATGTCAATCCGACTCTACTTTTCCTAAAAATT66340.17131335417809357No Hit
CCATACAGCCATGGAACAGGAACAGGATAGAAGTGCTGCAATCTATTCGA66170.1708743540241853No Hit
GCACCACATTCCCTTATACTGGAGATCCTCCATACAGCCATGGAACAGGA65230.1684469414084571No Hit
TGGTGGAACAGATCTTCATGATCTCAGAGAACTCTTCTTTCTTGATCCGT64710.16710411740826706No Hit
CAATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCA64530.16663929371589356No Hit
CTACTTTTCCTAAAAATTCCAGCGCAAAATGCCATAAGCACCACATTCCC61870.1597702324841521No Hit
AAATTAACCGGTCTCCTATATGAACTGCTAGGGAAAAATTTCTCGAATAG60080.15514781909888245No Hit
AGTAGAAACAAGGTCGTTTTTAAACAATTCGACACTAATTGATGGCCATC59780.15437311294492667No Hit
TCATATAGGAGACCGGTTAGCATGGTGGAGGCCATGGTGTCTAGGGCCCG59420.15344346556017968No Hit
GGCCTCCACCATGCTAGAAATTAACCGGTCTCCTATATGAACTGCTAGGG58880.15204899448305922No Hit
GTATGCTAGAGTCCCGTTTTCGTTTCATTACCAACACTACGTCCCCTTGC58770.1517649355599421No Hit
CCATGCTAACCGGTCTCCTATATGAACTGCTAGGGAAAAATTTCTCGAAT58630.1514034060214294No Hit
GAATATGATGCCGTTGCAACTACACATTCCTGGATTCCCAAGAGGAATCG58540.15117099417524266No Hit
ACATTCCCTTATACTGGAGATCCTCCATACAGCCATGGAACAGGAACAGG58200.1502929938674261No Hit
GAGTTCTTCAATGGTGGAACAGATCTTCATGATCTCAGAGAACTCTTCTT57840.14936334648267913No Hit
GAATAGAACGATTCCTCTTGGGAATCCAGGAATGTGTAGTTGCAACGGCA57550.14861446386718852No Hit
ATTTGAATGGATGTCAATCCGACTCTACTTTTCCTAAAAATTCCAGCGCA56940.1470392280208117No Hit
ACTCTACTTTTCCTAAAAATTCCAGCGCAAAATGCCATAAGCACCACATT55430.14313987371256748No Hit
AGCAGGTACTGATCCAAAATGGAAGACTTTGTGCGACAATGCTTCAATCC55290.14277834417405477No Hit
GTATGGATAGCAAATAGTAGCATTGCCACAACTACTTCAGTGCATGTGTG54660.1411514612507476No Hit
CACAGAGACTGGTGCACCCCAGCTCAACCCGATTGATGGACCACTACCTG52670.1360125770961741No Hit
CATATAGACTGTTGAATACAGATTTTGCCAGTAAGGTCCTGCACACTTTC51940.13412745878821497No Hit
GCATTTTTTCATGAAGGACAAGTTAAATTCATTATTTTTGCCGTCTGAGT50330.1299698690953188No Hit
ATGCTAGAAATTCCAACCGGTCTCCTATATGAACTGCTAGGGAAAAATTT50160.12953086894141053No Hit
TTTGAATGGATGTCAATCCGACTCTACTTTTCCTAAAAATTCCAGCGCAA49570.1280072801719641No Hit
GATGTCAATCCGACTCTACTTTTCCTAAAAATTCCAGCGCAAAATGCCAT49430.1276457506334514No Hit
GGTAATGCCAGCCCATGGTCCAGCCAAAAGCATGGAATATGATGCCGTTG48740.12586392647935304No Hit
TAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCACTCGCGAGATACTC48570.12542492632544475No Hit
CCTCCATACAGCCATGGAACAGGAACAGGATAGAAGTGCTGCAATCTATT48170.12439198478683701No Hit
ATCATATTCCATGCTTTTGGCTGGACCATGGGCTGGCATTACCACAGCAT47870.12361727863288122No Hit
CTCTTCTTTCTTGATCCGTCCAGACTCGAAGTCGACCCTGGCATCAATCC47130.12170633678645688No Hit
CATTTGGAAGTTTGTTTCATGTATTCGGATTTCCATTTCATCGACGAACG46630.12041515986319722No Hit
ATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCACT46130.11912398293993755No Hit
CTACCTGAGGATAATGAACCAAGTGGGTATGCACAAACAGACTGTGTTCT45820.11832345324751656No Hit
CCATATGGCCATAATCAAAAAGTACACATCAGGAAGGCAAGAGAAGAACC44650.11530209924708891No Hit
GTCCCGCACTCGCGAGATACTCACTAAGACCACTGTGGACCATATGGCCA44510.1149405697085762No Hit
CAATCCGACTCTACTTTTCCTAAAAATTCCAGCGCAAAATGCCATAAGCA44490.11488892263164581No Hit
GGTTAGCATGGTGGAGGCCATGGTGTCTAGGGCCCGGATTGATGCCAGGG44340.11450156955466792No Hit
TGTCAATCCGACTCTACTTTTCCTAAAAATTCCAGCGCAAAATGCCATAA44190.11411421647769002No Hit
ATGAAGGACAAGTTAAATTCATTATTTTTGCCGTCTGAGTTCTTCAATGG43660.11274556893903476No Hit
CCCCAGCTCAACCCGATTGATGGACCACTACCTGAGGATAATGAACCAAG43280.1117642744773574No Hit
CTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAGTAA43040.11114450955419276No Hit
CAAACAGACTGTGTTCTGTAAACAATGCTGTGGTAATGCCAGCCCATGGT43000.11104121540033199No Hit
GATCTTATCCTTTAAGAAATCTATTAGCCTTCCTGACTCATTAGCTGTTA42990.11101539186186679No Hit
ATATTCAATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCAGTC42970.11096374478493641No Hit
AACCATTTGAATGGATGTCAATCCGACTCTACTTTTCCTAAAAATTCCAG42510.1097758620155375No Hit
GCTAGAAATTAACCGGTCTCCTATATGAACTGCTAGGGAAAAATTTCTCG41370.10683197863050546No Hit
ACTGTGGACCATATGGCCATAATCAAAAAGTACACATCAGGAAGGCAAGA41140.106238037245806No Hit
ATTTTTGCCGTCTGAGTTCTTCAATGGTGGAACAGATCTTCATGATCTCA41080.10608309601501484No Hit
TATATGAACTGCTAGGGAAAAATTTCTCGAATAGATTGCAGCACTTCTGG40780.10530838986105903No Hit
GAACAGGAACAGGATAAAGTGGACGACAAACACAGAGACTGGTGCACCCC40620.10489521324561595No Hit
GAATGAAGTGGATGATGGCAATGAGATACCCAATTACAGCAGACAAGAGA40490.10455950724556844No Hit
AATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCAC40480.10453368370710323No Hit
ACACTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAG40330.10414633063012534No Hit
GAACTCTTCTTTCTTGATCCGTCCAGACTCGAAGTCGACCCTGGCATCAA40010.10331997739923912No Hit
ATACTGGAGATCCTCCATACAGCCATGGAACAGGAACAGGATACACCATG39450.10187385924518831No Hit
GTTCTTCAATGGTGGAACAGATCTTCATGATCTCAGAGAACTCTTCTTTC39060.10086674124504574No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTAGAA67500.0117.618131
AGTTGCG2250.0109.88585145
CGTATCG203.820875E-4109.07786145
TCTATCG607.2759576E-1296.9581145
GTAGAAA90800.087.498152
CGGGCCG2000.087.26228145
AGCGAAA8950.085.871061
TGACGCG850.085.55126145
GCAGGTA10550.084.513652
TAGAAAC98000.081.427993
CAGGTAC11850.075.8430563
ATCATAG3700.074.68394145
GACTGTA4550.073.51768145
GTAGTTA1500.067.87067145
AGAAACA118750.067.3155064
ACTGATC13800.067.2234658
GCGAAAG11800.066.96052
GAGCGAA2400.066.46231
CGAAAGC11850.066.0568543
GACAGTA19250.065.885797
Produced by FastQC (version 0.11.5)