FastQCFastQC Report
Sat 5 Dec 2020
H7KKVBGXH_n02_S6_R2_D01_FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7KKVBGXH_n02_S6_R2_D01_FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3946845
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCTTATACTGGAGATCCTCCATACAGCCATGGAACAGGAACAGGATACA291580.7387672938765013No Hit
TTTTTAAACAATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCG222950.5648815699628438No Hit
AATATATTCAATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCA216050.5473992518074564No Hit
GAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCACTCGCGAGATA199490.5054416882345265No Hit
GGTCAAATATATTCAATATGGAGAGAATAAAAGAACTGAGAGATCTAATG161950.41032774279202755No Hit
CACTAAGACCACTGTGGACCATATGGCCATAATCAAAAAGTACACATCAG153910.3899570416370544No Hit
CCTATATGAACTGCTAGGGAAAAATTTCTCGAATAGATTGCAGCACTTCT151230.38316680791873003No Hit
GTATGCTAGAGTCCCGTTTTCGTTTCATTACCAACACTACGTCCCCTTGC134140.3398663996179227No Hit
TTCTTGATCCGTCCAGACTCGAAGTCGACCCTGGCATCAATCCGGGCCCT127920.3241069765850952No Hit
AGTAGAAACAAGGCATTTTTTCATGAAGGACAAGTTAAATTCATTATTTT119790.30350824519331265No Hit
AGCAGGTACTGATCCAAAATGGAAGACTTTGTGCGACAATGCTTCAATCC116880.29613526753647534No Hit
GTAGAAACAAGGTCGTTTTTAAACAATTCGACACTAATTGATGGCCATCC116560.295324493361153No Hit
ATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCT116440.29502045304540714No Hit
TTTTTGGACAGTATGGATAGCAAATAGTAGCATTGCCACAACTACTTCAG113860.28848358625687104No Hit
GGATACACCATGGACACAGTAAACAGAACACACCAATACTCAGAAAAGGG112580.28524048955558173No Hit
AAACAAGGTCGTTTTTAAACAATTCGACACTAATTGATGGCCATCCGAAT107800.27312955031170466No Hit
GTCCAGACTCGAAGTCGACCCTGGCATCAATCCGGGCCCTAGACACCATG100540.25473511120907966No Hit
GTATGGATAGCAAATAGTAGCATTGCCACAACTACTTCAGTGCATGTGTG99590.25232812537609156No Hit
CAGTAAACAGAACACACCAATACTCAGAAAAGGGAAAGTGGACGACAAAC98360.2492117121396964No Hit
ATTCAATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCC95210.24123065385136735No Hit
ATCTAATGTCGCAGTCCCGCACTCGCGAGATACTCACTAAGACCACTGTG93590.23712610958879815No Hit
GTTAAATTCATTATTTTTGCCGTCTGAGTTCTTCAATGGTGGAACAGATC93100.23588461163283583No Hit
ACTGTGGACCATATGGCCATAATCAAAAAGTACACATCAGGAAGGCAAGA89570.22694075901131155No Hit
AATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAGTAAGT85980.21784488623191436No Hit
CTTCTTTCTTGATCCGTCCAGACTCGAAGTCGACCCTGGCATCAATCCGG85280.21607131772339677No Hit
CTTCAATGGTGGAACAGATCTTCATGATCTCAGAGAACTCTTCTTTCTTG84620.21439909598679452No Hit
CCATATGGCCATAATCAAAAAGTACACATCAGGAAGGCAAGAGAAGAACC82250.2083942997508136No Hit
GTGTAGTTGCAACGGCATCATATTCCATGCTTTTGGCTGGACCATGGGCT73720.18678210063987818No Hit
CCATACAGCCATGGAACAGGAACAGGATACACCATGGACACAGTAAACAG73630.1865540704030688No Hit
GAATAGAACGATTCCTCTTGGGAATCCAGGAATGTGTAGTTGCAACGGCA73140.1853125724471065No Hit
ATACTGGAGATCCTCCATACAGCCATGGAACAGGAACAGGATACACCATG70450.1784970020358033No Hit
CACCAATACTCAGAAAAGGGAAAGTGGACGACAAACACAGAGACTGGTGC69350.1757099658081328No Hit
GATCTTCATGATCTCAGAGAACTCTTCTTTCTTGATCCGTCCAGACTCGA67490.17099734091407187No Hit
CTGTGGACCATATGGCCATAATCAAAAAGTACACATCAGGAAGGCAAGAG66820.1692997824844908No Hit
CAATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCA64050.162281518529357No Hit
CTACGTCCCCTTGCCCAATTAGCACATTAGCCTTCTCTCCTTTTGCAAGA64020.16220550845042053No Hit
CACTACGTCCCCTTGCCCAATTAGCACATTAGCCTTCTCTCCTTTTGCAA63340.1604826133278606No Hit
GTCCCGCACTCGCGAGATACTCACTAAGACCACTGTGGACCATATGGCCA61790.15655542591614316No Hit
CATATAGACTGTTGAATACAGATTTTGCCAGTAAGGTCCTGCACACTTTC61720.1563780690652914No Hit
AGTAGAAACAAGGTCGTTTTTAAACAATTCGACACTAATTGATGGCCATC60100.15227352480272216No Hit
GTCTATATGCGTCTCCACAACTTGAGGGGTTTTCGGCTGAATCTAGAAAA59600.15100669015378104No Hit
CTCCTATATGAACTGCTAGGGAAAAATTTCTCGAATAGATTGCAGCACTT59350.1503732728293105No Hit
GAATAAGAGAGGCTATCTAATAAGAGCACTGACATTAAATACGATGACCA58910.14925845833824233No Hit
CTTCTGGTACATCTGTTCATCCTCAAGAATTCCCCTTTGGCTTGTGTTGA58530.14829566400504707No Hit
GGCCTCCACCATGCTAGAAATTCCAACCGGTCTCCTATATGAACTGCTAG58160.14735820636483066No Hit
TTATAGGTGCCATGCTCAGGATGTTTCTGAACCACTCGGGTTGATTTCTG57160.14482453706694842No Hit
CATTTGGAAGTTTGTTTCATGTATTCGGATTTCCATTTCATCGACGAACG56680.14360837580396493No Hit
GTATATTGTCTTGGTGTATTTCTTTTGTCCAAGATTCAGTATCGAGACTC56630.14348169233907082No Hit
GTCAGTAAGTATGCTAGAGTCCCGTTTTCGTTTCATTACCAACACTACGT54350.13770492633989934No Hit
TAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCACTCGCGAGATACTC52440.1328656179809443No Hit
CTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAGTAA51700.1309907027005114No Hit
CTATTAGAAGAGGCCTTATTTTCTCAATTTTCTTCTTTGTTGATTCATTG50740.1285583801745445No Hit
CCATTTGAATGGATGTCAATCCGACTCTACTTTTCCTAAAAATTCCAGCG50730.12853304348156563No Hit
ATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCACT50550.12807698300794684No Hit
TCCCAGGACTCAGTGATGCTGTGCCATCTATTAGAAGAGGCCTTATTTTC50230.12726620883262454No Hit
AACTTATACAATATACGGAATCTTCACATTCCTGAAGTCTGCTTAAAATG49940.1265314447362387No Hit
CATCAGGAAGGCAAGAGAAGAACCCCGCACTCAGAATGAAGTGGATGATG49890.1264047612713446No Hit
CCACTATATGATGCAATCAAATGCATGAAGACATTCTTTGGCTGGAAAGA49540.1255179770170858No Hit
AAACAGAACACACCAATACTCAGAAAAGGGAAAGTGGACGACAAACACAG49170.12458051937686938No Hit
CTTTATGACTGACAAAGGGATTCAGGGGATTACAAAGTCTTCCCCGATAA49100.12440316252601762No Hit
CCTCCATACAGCCATGGAACAGGAACAGGATACACCATGGACACAGTAAA49030.12422580567516588No Hit
GTATAAGTTTGGTCCTCCATCTGATACTAATAGCCCTACCTTTGATTGGG48790.12361772504367412No Hit
GGACAAGTTAAATTCATTATTTTTGCCGTCTGAGTTCTTCAATGGTGGAA48360.12252824724558477No Hit
ACACTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAG47610.12062799527217309No Hit
CTCTTATTCAGTCTTTGTTTTTTCTTCCCTATTGTTCTTTGCGTGACCAT47560.12050131180727898No Hit
GACCACTGTGGACCATATGGCCATAATCAAAAAGTACACATCAGGAAGGC47520.1203999650353637No Hit
CTAAGACCACTGTGGACCATATGGCCATAATCAAAAAGTACACATCAGGA47090.11931048723727432No Hit
ATATACAACTTGAGGGGTTTTCGGCTGAATCTAGAAAATTGCTTCTCATT46820.11862639652684612No Hit
CTTCTAATAGATGGCACAGCATCACTGAGTCCTGGGATGATGATGGGCAT46650.11819567274620615No Hit
CATTAGCCTTCTCTCCTTTTGCAAGATTGCTCAGTTCATTGATGCTTAAT46260.11720754172003207No Hit
TTACCAACACTACGTCCCCTTGCCCAATTAGCACATTAGCCTTCTCTCCT46030.11662479778151917No Hit
GATCTAATGTCGCAGTCCCGCACTCGCGAGATACTCACTAAGACCACTGT45630.11561133006236628No Hit
CCACATTCCCTTATACTGGAGATCCTCCATACAGCCATGGAACAGGAACA45450.11515526958874747No Hit
TCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAGTAAGTATGCTAGAGTCC44740.11335636438725108No Hit
AAACAAGGCATTTTTTCATGAAGGACAAGTTAAATTCATTATTTTTGCCG44530.1128242938346958No Hit
ATATTCAATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCAGTC43970.11140543902788178No Hit
ATGGAAGACTTTGTGCGACAATGCTTCAATCCAATGATCGTCGAGCTTGC43510.11023995115085594No Hit
CCTATAATGTTCTCAAACAAAATGGCAAGACTAGGGAAAGGGTACATGTT43270.1096318705193642No Hit
ATACTCACTAAGACCACTGTGGACCATATGGCCATAATCAAAAAGTACAC42300.10717421130041845No Hit
CCACTACCTGAGGATAATGAACCAAGTGGGTATGCACAAGTAAACAATGC42290.1071488746074396No Hit
GTCCTGGGATGATGATGGGCATGTTCAACATGCTAAGTACGGTCTTGGGA42220.10697151775658786No Hit
GTCTGGCTGTCAGTAAGTATGCTAGAGTCCCGTTTTCGTTTCATTACCAA41590.10537530609892205No Hit
ATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAGTAAGTA40970.10380443113423507No Hit
AAATAAGACAGGGACATTTGAATTCACAAGCTTTTTTTATCGCTATGGAT40660.10301899365189157No Hit
GAACAGATCTTCATGATCTCAGAGAACTCTTCTTTCTTGATCCGTCCAGA40610.10289231018699746No Hit
CTCCAAAGCTGGGTAGCTCCATGCTAAAATTAGCCACAAATCCATAGCGA40350.10223355616954807No Hit
AATTAGCACATTAGCCTTCTCTCCTTTTGCAAGATTGCTCAGTTCATTGA39850.10096672152060697No Hit
CCACTACACATTCCTGGATTCCCAAGAGGAATCGTTCTATTCTCAACACA39620.10038397758209405No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCAGGTA18050.0108.4263462
AGTAGAA30700.0107.6923451
CCGCACG3250.0107.39513145
CCGACGG501.9099389E-10101.80163145
TGGACCG2850.099.505356145
CAGGTAC19800.098.815643
CGCCGTG406.013652E-790.89431145
CTCAACG651.4551915E-1189.49593145
GGTACTG22250.088.900615
CGTAACG259.278788E-487.25854145
AGTTCCG950.084.19683145
CGTATCG353.3882974E-583.10337145
ACTTCCG353.3882974E-583.10337145
TGCCGAG702.910383E-1183.10337145
AGCGAAA6200.080.689261
AGATCGG1550.079.752426145
GATTACG755.4569682E-1177.56314145
ACTGATC25950.076.7960748
AAATATA35650.075.4026265
GTACTGA27750.073.115796
Produced by FastQC (version 0.11.5)