FastQCFastQC Report
Sat 5 Dec 2020
H7KKVBGXH_n02_S6_R2_C03_FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7KKVBGXH_n02_S6_R2_C03_FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2627773
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTTAGAGCTATTGATGTGTTTTTTCATCTAATTTGGAAAATTTTAGCAA41600.15830895591057523No Hit
CAATAGGAGAACCAAAAATATGAAAGAACTGAATATAACTTCTAGGACTA41010.15606370869934352No Hit
GTGTACACCCAGCCTTTGAACATTTTTATCTGTGGCTGCATTCATGCCAG31170.1186175518204959No Hit
CTTTAGTGATCTGTGGAATAACATCAAGAGACCTATCATTTGGTATAACT30780.11713340535883428No Hit
GTTCAATGGCTGAGCCAAGCCTTGAATGGCATGTCAGGTAGGCCAGGAAA28920.1100551683878326No Hit
GTTCAAAGGCGCGCAAAGTAGAGCAGTACTGTGTTCCCATTCGGTACCCC28560.10868518703860644No Hit
CACACAGGAGCTATCAATCACTCTCGTCTCCCTGGTTTCTGTATGCACTC28290.10765770102668686No Hit
GCTCTAAGAAGCTCAATGAATTCCACTAAGGGTAAATATAAAAAAACGAC26550.10103612450542722No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATCG151.2153013E-4145.44513145
ACGAACG1650.079.3337145
TAGATCG1102.5465852E-1159.500275145
TGACGCG1700.055.61137145
AGCGAAA5150.054.8994751
CCGACGG1901.2551027E-1038.275032145
GCGAAAG7900.035.7834472
CGAAAGC8050.035.1099933
GCTACCG1458.499319E-735.10744145
CGAACTG2501.8189894E-1234.906826145
TAAGTCG2950.031.9368139
CTATTGA20200.031.9307339
TAGTCGT950.00473762430.5145249
GTATACC6900.030.4580844
GCTATTG20950.030.095778
GCCGACC2202.2006134E-829.750137145
GTAGAAA17650.029.5732171
ACCGTGC12450.028.5231158
TGTCTCG3351.8189894E-1228.220697145
GCATTAA14300.027.8828681
Produced by FastQC (version 0.11.5)