FastQCFastQC Report
Sat 5 Dec 2020
H7KKVBGXH_n02_S6_R2_C01_FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7KKVBGXH_n02_S6_R2_C01_FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2425589
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGCAGGTACTGATCCAAAATGGAAGACTTTGTGCGACAATGCTTCAATCC64420.26558497750443294No Hit
TTTTTGGACAGTATGGATAGCAAATAGTAGCATTGCCACAACTACTTCAG54300.22386315241370242No Hit
GTATGGATAGCAAATAGTAGCATTGCCACAACTACTTCAGTGCATGTGTG45550.18778944000817946No Hit
GTATTATACATCTGAAACTAATGTAACATTGTGTGTCCACTATCATCAAT38520.15880678878408502No Hit
GTCCCTAAGTGCCTGAACAATGAGAAGCAATTTTCTAGATTTCTCACATA37820.15592089179164317No Hit
CAATAGGAGAACCAAAAATATGAAAGAACTGAATATAACTTCTAGGACTA30830.12710314896711686No Hit
CTATTGAAGCACCGATTTGAGATAATTGAAGGAAGAGACCGAATCATGGC29580.12194976148061358No Hit
CTTATAGACTTGTCTAATCACTGTATTATACATCTGAAACTAATGTAACA27770.11448765640015683No Hit
GTGGTAAGACAAGAAATGGCCAGTAGGAGTCTATGGGATTCCTTTCGTCA27420.11304470790393592No Hit
ATGGAAGACTTTGTGCGACAATGCTTCAATCCAATGATCGTCGAGCTTGC26450.10904567921440937No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGACGCG554.3004547E-879.344536145
CGTATCG350.003522156562.342136145
ACTGATC17350.056.3899738
AGCGAAA3950.055.0791631
GCGAAAG5850.049.586941
CAGGTAC19000.049.2074173
GGTACTG20050.047.3524485
GCAGGTA19700.046.3686182
CGAAAGC6500.043.498182
GTACTGA24000.042.2759446
GACAGTA19500.041.99727
CCGACGG700.0010404241.561424145
GACCGTC1553.4851837E-837.4054457
TACTGAT26200.036.7897537
CTTACCG800.002005419736.36625145
ACAGTAT31800.034.868398
CAGTATG25100.033.7815639
TTGTCCG1102.3917851E-433.060226145
ATTAGCG900.003572780932.325554145
GGATAGC20450.031.1870885
Produced by FastQC (version 0.11.5)